Porcine reproductive and respiratory syndrome vaccine virus
View Patent ↗The present invention relates to modified, live Porcine Reproductive and Respiratory Syndrome viruses. Viruses were genetically analyzed and selected based on phylogenetic grouping for modification by repeated passage in tissue culture. The modified, live viruses were assessed for the ability to provide protective immunity to heterologous viruses. The modified, live viruses are useful in vaccines, particularly in vaccines which can treat infection of swine by multiple heterologous viruses.
1. A modified, live Porcine Reproductive and Respiratory Syndrome (PRRS) virus strain, wherein the consensus complementary DNA sequence of said PRRS strain is at least 95% identical to a sequence selected from the group of SEQ ID NO: 4, SEQ ID NO: 10, and SEQ ID NO: 11.
2. The modified, live PRRS virus strain of claim 1 , wherein the consensus complementary DNA sequence of said PRRS strain is at least 98% identical to a sequence selected from the group of SEQ ID NO: 4, SEQ ID NO: 10, and SEQ ID NO: 11.
3. The modified, live PRRS virus strain of claims 1 , wherein said PRRS strain is ND 99-14.
4. The PRRS strain of claim 1 , wherein said PRRS virus strain is passaged at least 80 times in tissue culture cells.
5. The PRRS strain of claim 1 , wherein said PRRS virus strain is passaged 100 times in tissue culture cells.
6. An immunogenic composition comprising a modified, live PRRS virus strain having a consensus complementary DNA sequence at least 95% identical to a sequence selected from the group of SEQ ID NO: 4, SEQ ID NO: 10, and SEQ ID NO: 11; and
a pharmaceutically-acceptable excipient, stabilizer, solubilizer, or diluent.
7. The immunogenic composition of claim 6 , wherein the consensus complementary DNA sequence is at least 98% identical to a sequence selected from the group of SEQ ID NO: 4, SEQ ID NO: 10, and SEQ ID NO: 11.
8. The immunogenic composition of claim 6 , wherein the modified, live PRRS virus strain is ND 99-14.
9. The immunogenic composition of claim 6 , further comprising an adjuvant.
10. A method of treating or preventing a symptom caused by type 2 Porcine Reproductive and Respiratory Syndrome (PRRS) in a porcine animal, comprising administering to said porcine animal an immunogenic composition comprising a modified, live PRRS virus strain having a consensus complementary DNA sequence at least 98% identical to a sequence selected from the group of SEQ ID NO: 4, SEQ ID NO: 10, and SEQ ID NO: 11; and
a pharmaceutically-acceptable excipient, stabilizer, solubilizer, or diluent.
11. A vaccine comprising a modified, live PRRS virus strain, wherein said PRRS virus strain is ND 99-14.
12. A method of treating or preventing a symptom caused by type 2 Porcine Reproductive and Respiratory Syndrome (PRRS) virus infection in a porcine animal, comprising administering to said porcine animal a vaccine comprising a modified, live PRRS virus strain, wherein said PRRS virus strain is ND 99-14.
13. The method of claim 12 , wherein the PRRS virus infection is caused by a virulent type 2 PRRS virus heterologous to the modified, live PRRS virus strain in the vaccine.