High capacity diketopiperazine microparticles and methods
View Patent ↗Disclosed herein are diketopiperazine microparticles having high capacity for adsorbing a drug or active agent. In particular, the diketopiperazine microparticle are formed using fumaryl diketopiperazine and can comprise a drug in large doses for the treatment of disease or disorders by pulmonary delivery via oral inhalation.
1. A method of delivering a drug or an active agent to a patient in need thereof comprising administering to the patient in need thereof using a reusable inhaler an inhalable composition comprising a drug or active agent and diketopiperazine microparticles, each of the diketopiperazine microparticles comprising a plurality of structural domains, each of the plurality of structural domains comprising a nucleus surrounded by layers of a porous crystalline material, and each of the diketopiperazine microparticles have a mass-weighted average particle size of about 33,000 to about 216,000 voxels measured by X-ray tomography for particle sizes measuring from about 0.5 pm to about 4 pm in geometric diameter.
2. The method of claim 1 , wherein said diketopiperazine is fumaryl diketopiperazine or 3,6-bis(N-fumaryl-4-aminobutyl)-2,5-diketopiperazine; or salt thereof.
3. The method of claim 1 , wherein the diketopiperazine microparticles comprise structural domains ranging in number-weighted size from about 250 voxels to about 1,400 voxels.
4. The method of claim 1 , wherein the diketopiperazine microparticles comprise structural domains ranging in voxel-weighted size from about 500 voxels to about 2,000 voxels.
5. The method of claim 1 , wherein the diketopiperazine microparticles have a specific surface area greater than 70 m 2 /g.
6. The method according to claim 1 , further comprising a surfactant.
7. The method according to claim 6 , wherein said surfactant is polysorbate 80.
8. The method of claim 1 , wherein said drug or active agent is a small organic molecule, peptide or protein, a lipid, or a nucleic acid molecule, or combinations thereof.
9. The method according to claim 1 , wherein said active agent is insulin.
10. The method of claim 8 , wherein said peptide or protein is insulin, parathyroid hormone, calcitonin, glucagon, glucagon-like peptide 1, oxyntomodulin, oxytocin, CCK-8, PYY3-36, ghrelin, vasoactive intestinal peptide, leuprolide, growth hormone, RGD (Arg-Gly-Assp) peptide, growth hormone releasing peptide, DDAVP (desamino-Cys-1, D-arg8) vasopressin peptide, cyclosporine, detirelex, somatostatin, interferon-.alpha., granulocyte colony stimulating factor, IgG, an analog or active fragment thereof.
11. The method of claim 8 , wherein the small organic molecule is a neurotransmitter agonist, a neurotransmitter antagonist, a pain inhibitory agent, a vaccine, an anti-inflammatory agent, an anti-cancer agent, a cell receptor agonist molecule, cell receptor antagonist molecule, an immunosuppressant, a vasodilator, a statin or an anti-infective agent.
12. The method of claim 1 , wherein the inhalable composition is in dry powder form.
13. A dry powder inhaler for use with the method according to claim 1 , and wherein said inhalable composition is in dry powder form.
14. The dry powder inhaler of claim 13 , wherein the inhalable composition is provided in a unit dose cartridge for using with a reusable inhaler.