Catalysis of diketopiperazine synthesis
Provided is a method for the synthesis of N-protected 3,6-bis-[4-aminobutyl]-2,5-diketopiperazine including the step of heating a solution of ε-amino protected lysine in the presence of a catalyst selected from the group consisting of sulfuric acid, phosphoric acid, and phosphorus pentoxide.
1. A method for the synthesis of an N-protected 3,6-bis[4-aminoalkyl]-2,5-diketopiperazine comprising heating a solution comprising:
a. providing an amino acid having a free α-amine group and a protected amine group, and
b. means for catalyzing dehydrative cyclocondensation of said amino acid.
2. The method of claim 1 wherein said the solution is heated to a target temperature and the target temperature is achieved in about 4-6 hours.
3. The method of claim 1 , wherein said solution is heated to a target temperature and the target temperature is maintained for up to 6 hours.
4. The method of claim 1 , wherein said solution is heated to a target temperature and wherein said synthesis is substantially complete within about 8 hours of reaching said target temperature.
5. The method of claim 1 , wherein the solution is heated to a target temperature of between 160° C. and 170° C.
6. The method of claim 1 , wherein said means for catalyzing dehydrative cyclocondensation of said amino acid comprises addition of a catalyst to the solution, wherein said catalyst comprises at least one of sulfuric acid, phosphoric acid, or phosphorus pentoxide.
7. The method of claim 1 , wherein the solution further comprises m-cresol, ethylene glycol, propylene glycol, toluene, or xylene.
8. The method of claim 1 , further comprising washing a precipitate formed upon incubation with deionized water.