Observation device and focus adjustment method
View Patent ↗An observation device, comprising an image sensor that forms images of a specimen and outputs an image signal, an AF detection circuit that calculates evaluation values based on the image signal, and a focus control circuit that controls focus position based on the evaluation values, wherein the AF detection circuit calculates a plurality of evaluation values based on signals relating to a plurality of frequency bands of the image signal, and the focus control circuit controls focus position based on maximum value or minimum value of the plurality of evaluation values.
1. An observation device, comprising:
an image sensor that images a specimen and outputs an image signal,
an AF detection circuit that calculates a focus evaluation value showing a larger value as degree of focus increases, based on the image signal, and
a focus control circuit that changes focus position of the image sensor and controls focus position based on the focus evaluation value,
wherein
the AF detection circuit calculates a plurality of evaluation values based on signals relating to a plurality of frequency bands of the image signal, and
the focus control circuit controls focus position based on maximum value or minimum value of the plurality of evaluation values corresponding to change in the focus position.
2. The observation device of claim 1 , wherein:
the focus control circuit controls focus position by selecting focus position corresponding to a maximum value or minimum value of the plurality of evaluation values.
3. The observation device of claim 2 , wherein:
when respective minimum values are detected in the plurality of evaluation values, the focus control circuit selects a focus position based on rate of change of the evaluation values corresponding to respective minimum values.
4. The observation device of claim 3 , wherein:
rate of change of the evaluation values is a reduction rate from a peak to a minimum value of a plurality of evaluation values, or increase rate from a minimum value to a peak.
5. The observation device of claim 3 , wherein:
rate of change of the evaluation values is an absolute value of inclination from a peak to a minimum value of the plurality of evaluation values, or inclination from a minimum value to a peak.
6. The observation device of claim 3 , wherein:
the focus control circuit selects a focus position corresponding to a minimum value having the largest rate of change of the evaluation values corresponding to respective minimum values of the plurality of evaluation values.
7. The observation device of claim 1 , wherein:
in a case where, among the plurality of evaluation values, there is not a minimum value of evaluation value relating to a higher frequency than the low frequency, within a given range from a focus position corresponding to minimum value of evaluation value relating to a low frequency, the focus control circuit selects a focus position corresponding to a minimum value of evaluation value relating to the low frequency.
8. A focus adjustment method for an observation device that has an image sensor that forms images of a specimen, comprising:
changing focus position of the image sensor, and calculating a focus evaluation value showing a larger value as degree of focus increases, based on an image signal output by the image sensor,
when calculating the focus evaluation value, calculating a plurality of evaluation values based on signals relating to a plurality of frequency bands of the image signal, and
controlling focus position based on maximum value or minimum value of the plurality of evaluation values corresponding to change in the focus position.
9. The focus adjustment method of claim 8 , further comprising:
controlling focus position by selecting focus position corresponding to a maximum value or minimum value of the plurality of evaluation values.
10. The focus adjustment method of claim 9 , further comprising:
when retrospective minimum values are detected in the plurality of evaluation values, selecting a focus position based on rate of change of the evaluation values corresponding to respective minimum values.
11. The focus adjustment method of claim 10 , wherein:
rate of change of the evaluation values is a reduction rate from a peak to a minimum value of a plurality of evaluation values, or increase rate from a minimum value to a peak.
12. The focus adjustment method of claim 10 , wherein:
rate of change of the evaluation values is an absolute value of inclination from a peak to a minimum value of the plurality of evaluation values, or inclination from a minimum value to a peak.
13. The focus adjustment method of claim 10 , further comprising:
selecting a focus position corresponding to a minimum value having the largest rate of change of the evaluation values corresponding to respective minimum values of the plurality of evaluation values.
14. The focus adjustment method of claim 8 , further comprising:
in a case where, among the plurality of evaluation values, there is not a minimum value of evaluation value relating to a higher frequency than the low frequency, within a given range from a focus position corresponding to minimum value of evaluation value relating to a low frequency, selecting a focus position corresponding to a minimum value of evaluation value relating to the low frequency.
15. A non-transitory computer-readable medium storing a processor executable code, which when executed by at least one processor which is included within an observation device that has an image sensor for forming an image of a specimen, performs a focus adjustment method, the focus adjustment method comprising:
changing focus position of the image sensor, and calculating a focus evaluation value exhibiting a larger value as degree of focus increases, based on an image signal output by the image sensor,
when calculating the focus evaluation value, calculating a plurality of evaluation values based on signals relating to a plurality of frequency bands of the image signal, and
controlling focus position based on maximum value or minimum value of the plurality of evaluation values corresponding to change in the focus position.
16. The non-transitory computer-readable medium of claim 15 , wherein the focus adjustment method further comprises:
controlling focus position by selecting focus position corresponding to a maximum value or minimum value of the plurality of evaluation values.
17. The non-transitory computer-readable medium of claim 16 , wherein the focus adjustment method further comprises:
when retrospective minimum values are detected in the plurality of evaluation values, selecting a focus position based on rate of change of the evaluation values corresponding to respective minimum values.
18. The non-transitory computer-readable medium of claim 17 , wherein the focus adjustment method further comprises:
rate of change of the evaluation values is a reduction rate from a peak to a minimum value of a plurality of evaluation values, or increase rate from a minimum value to a peak.
19. The non-transitory computer-readable medium of claim 17 , wherein the focus adjustment method further comprises:
rate of change of the evaluation values is an absolute value of inclination from a peak to a minimum value of the plurality of evaluation values, or inclination from a minimum value to a peak.
20. The non-transitory computer-readable medium of claim 17 , wherein the focus adjustment method further comprising
selecting a focus position corresponding to a minimum value having the largest rate of change of the evaluation values corresponding to respective minimum values of the plurality of evaluation values.