Compositions to detect Atopobium vaginae nucleic acid
The disclosed invention include nucleic acid oligomers that may be used as amplification oligomers, including primers, as capture probes for sample preparation, and detection probes for detection of 16S rRNA from Atopobium vaginae in samples by using methods of specific nucleic acid amplification and detection.
1. A composition comprising at least two amplification oligomers for amplifying a 16S rRNA of A. vaginae or a gene encoding 16S rRNA of A. vaginae , wherein said amplification oligomers are configured to specifically hybridize to said 16S rRNA of A. vaginae or a gene encoding 16S rRNA of A. vaginae and generate an amplicon comprising a nucleotide sequence that is SEQ ID NO:43, wherein at least one of the amplification oligomers comprises a target-specific sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:27, and wherein at least one of the amplification oligomers comprises a 5′ promoter sequence, and
wherein the composition further comprises a detection probe oligomer comprising (i) a nucleotide sequence of from 10 nucleotides in length to 40 nucleotides in length configured to specifically hybridize to all or a portion of a region of a target sequence of an A. vaginae 16S rRNA nucleic acid or amplified nucleic acid sequence, said region corresponding to SEQ ID NO:44; and (ii) a label selected from a luminescent compound and a fluorophore.
2. The composition of claim 1 , wherein a first oligomer member of the at least two amplification oligomers comprises the target-specific sequence of SEQ ID NO:2 and a second oligomer member of the at least two amplification oligomers comprises the target-specific sequence of SEQ ID NO:27.
3. The composition of claim 2 , wherein the second oligomer member comprises the sequence of SEQ ID NO:3.
4. The composition of claim 1 , wherein said promoter sequence is a T7 RNA polymerase promoter sequence.
5. The composition of claim 4 , wherein said promoter sequence is SEQ ID NO:25.
6. The composition of claim 5 , wherein at least one of the amplification oligomers comprises the sequence of SEQ ID NO:3.
7. The composition of claim 1 , wherein the detection probe comprises a sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:15, SEQ ID NO:16, and SEQ ID NO:17.
8. The composition of claim 1 , wherein the label is a chemiluminescent label.
9. The composition of claim 1 , wherein said region corresponds to SEQ ID NO:45.
10. The composition of claim 1 , wherein said region corresponds to SEQ ID NO:46.
11. The composition of claim 1 , wherein said detection probe oligomer is from 11 nucleotides in length to 29 nucleotides in length, contains a sequence corresponding to SEQ ID NO:48, and specifically hybridizes to all or a portion of a region of an A. vaginae nucleic acid or amplified nucleic acid sequence corresponding to SEQ ID NO:44.
12. The composition of claim 1 , wherein the sequence of said detection probe oligomer is SEQ ID NO:4.
13. A kit comprising at least two amplification oligomers for amplifying a 16S rRNA of A. vaginae or a gene encoding 16S rRNA of A. vaginae , wherein said amplification oligomers are configured to specifically hybridize to said 16S rRNA of A. vaginae or a gene encoding 16S rRNA of A. vaginae and generate an amplicon comprising a nucleotide sequence that is SEQ ID NO:43, wherein at least one of the amplification oligomers comprises a target-specific sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:27, and wherein at least one of the amplification oligomers comprises a 5′ promoter sequence, and
wherein the kit further comprises a detection probe oligomer comprising (i) a nucleotide sequence of from 10 nucleotides in length to 40 nucleotides in length configured to specifically hybridize to all or a portion of a region of a target sequence of an A. vaginae 16S rRNA nucleic acid or amplified nucleic acid sequence, said region corresponding to SEQ ID NO:44; and (ii) a label selected from a luminescent compound and a fluorophore.
14. The kit of claim 13 , wherein a first oligomer member of the at least two amplification oligomers comprises the target-specific sequence of SEQ ID NO:2 and a second oligomer member of the at least two amplification oligomers comprises the target-specific sequence of SEQ ID NO:27.
15. The kit of claim 14 , wherein the second oligomer member comprises the sequence of SEQ ID NO:3.
16. The kit of claim 13 , wherein said promoter sequence is a T7 RNA polymerase promoter sequence.
17. The kit of claim 16 , wherein said promoter sequence is SEQ ID NO:25.
18. The kit of claim 17 , wherein at least one of the amplification oligomers comprises the sequence of SEQ ID NO:3.