Thermal cycler
A system for thermal cycling, comprising at least one separate vessel holder for taking up a reaction vessel providing a contact surface for the reaction vessel, wherein the vessel holder is surrounded by individual liquid channels for cooling and at least one electrical heater is attached to the at least one separate vessel holder; and a multilayer liquid port for attachment of at least one vessel holder for providing the cooling liquid to the at least one separate vessel holder, and at least one control unit for connecting with the at least one electrical heater.
1. A system for thermal cycling, comprising:
at least one separate vessel holder for taking up a single reaction vessel and providing a contact surface for the single reaction vessel, wherein each of the at least one separate single vessel holder comprises:
an internal individual vertical meandering fluidic path in a descending flow path around the reaction vessel, configured for supplying a cooling liquid or pressurized air on one side of said at least one separate vessel holder from its upper to its lower side and on its opposite side from its lower to its upper side; and
at least one individual electrical heater attached on opposite sides next to the at least one separate vessel holder for individually adjusting the temperature of the single reaction vessel;
a multilayer liquid port with at least one sealing contact surface for the at least one separate vessel holder and inlet and outlet openings for the attachment of the at least one separate vessel holder configured for providing the cooling liquid or the pressurized air to the at least one separate vessel holder;
a 3-port/2-way control valve inside the multilayer liquid port, such control valve connected to (i) a first membrane valve connected to liquid and (ii) a second membrane valve connected to pressurized air, wherein the first and second membrane valves are 2-port/2-way valves and are alternatingly switched on and off by the control valve and the default position of the control valve is pressurized air; and
at least one control unit for connecting with the at least one electrical heater.
2. The system of claim 1 , wherein said at least one separate vessel holder has an optical path access area at its lower side.
3. The system of claim 2 , wherein the optical path access area of the at least one separate vessel holder has a ventilation channel for its cleaning.
4. The system of claim 1 , wherein the at least one separate vessel separate holder has an inlet and an outlet for the cooling liquid or pressurized air at the upper side of the at least one vessel holder.
5. The system of claim 1 , wherein liquid channel of the fluidic path of the at least one separate vessel holder has an acceptance for a maximum of two of the at least one electrical heater at opposite sides.
6. The system of claim 1 , wherein the at least one control unit is a printed circuit board (PCB).
7. The system of claim 6 , wherein the printed circuit board (PCB) is connected with at least one and a maximum of eight of the at least one separate vessel holder and at least one electrical heater, wherein four of the at least one separate vessel holder attached to at least one electrical heating form a unit.
8. The system of claim 1 , wherein the multilayer port has at least one control valve configured for providing the cooling liquid or the pressurized air to each of the at least one separate vessel holder.
9. The system of claim 1 , wherein the at least one separate vessel holder has stands for attachment to the printed circuit board (PCB).
10. The system of claim 1 , wherein the at least one electrical heating has electrical connections for attachment to electrical connections on the PCB for transmission of electricity and controlling the at least one electrical heater.
11. The system of claim 1 , wherein four of the at least one separate vessel holder, one multilayer port and one PCB form a unit.
12. The system of claim 1 , further comprising an optical detection device.
13. The system of claim 1 , wherein the optical detection device comprises for each of the at least one separate vessel holder one optical fiber and a dichroic mirror for excitation and emission path.