Methods and compositions for polymer superparamagnetic particles for nucleic acid extraction
A composition is described comprising superparamagnetic particles. Optionally, the particles comprise microbeads. Optionally, the particles comprise nanobeads. Optionally, the particles are non-spherical particles. Optionally, the particles are non-spherical particles suitable for RNA or DNA extraction. A method is described for forming microparticles. Optionally, the method comprises using citrate precipitation. Optionally, a kit comprising one or more of the particles is described. Optionally, a kit for sample preparation for nucleic acid extraction is described comprising non-spherical microparticles or nanoparticles.
1. A method of forming microparticles comprising:
providing a plurality of polystyrene micron particles;
coating each of the polystyrene micron particles with an epoxy layer;
forming an amine surface on the epoxy layer;
suspending the polystyrene micron particles having the amine surface in an aqueous solution to provide a particle suspension;
adding the particle suspension into a dissolved ferric and ferrous chloride solution;
allowing the ferric and ferrous chloride from the solution to penetrate inside the polystyrene micron particles using the amine surface hydrophilicity; and
precipitating the ferric and ferrous ions inside the particles using ammonia; and
wherein the method steps are carried out without using toluene.
2. A method of forming microparticles comprising:
providing a plurality of polystyrene micron particles;
coating each of the polystyrene micron particles with an epoxy layer;
forming an amine surface on the epoxy layer;
suspending the polystyrene micron particles having the amine surface in an aqueous solution to provide a particle suspension;
adding the particle suspension into a dissolved ferric and ferrous chloride solution;
allowing the ferric and ferrous chloride from the solution to penetrate inside the polystyrene micron particles using the amine surface hydrophilicity;
precipitating the ferric and ferrous ions inside the particles using ammonia;
forming an aldehyde activated surface on the amine surface; and
reacting the aldehyde activated surface with protein lysine side chain to form streptavidin conjugated beads; and
wherein the method steps are carried out without using toluene.
3. A method of forming microparticles comprising:
providing a plurality of polystyrene micron particles;
coating each of the polystyrene micron particles with an epoxy layer;
forming an amine surface on the epoxy layer;
suspending the polystyrene micron particles having the amine surface in an aqueous solution to provide a particle suspension;
adding the particle suspension into a dissolved ferric and ferrous chloride solution;
allowing the ferric and ferrous chloride from the solution to penetrate inside the polystyrene micron particles using the amine surface hydrophilicity; and
precipitating the ferric and ferrous ions inside the particles using ammonia;
forming an aldehyde activated surface on the amine surface without drying the polystyrene micron particles after forming said precipitate therein; and
wherein the method steps are carried out without using toluene.