Animal protein-free media for cultivation of cells
The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
1. A method for cultivating cells, comprising the steps of:
(a) providing an animal protein-free cell culture medium;
(b) adding a supplement to the animal protein-free cell culture medium comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the resultant concentration of the at least one polyamine in the animal protein-free cell culture medium ranges from about 0.5 to about 30 mg/L; and
(c) propagating the cells in the animal protein-free cell culture medium to form a cell culture.
2. The method according to claim 1 , wherein the cells are selected from the group consisting of mammalian cells, insect cells, avian cells, bacterial cells, and yeast cells.
3. The method according to claim 2 , wherein the mammalian cells are CHO cells.
4. The method according to claim 3 , wherein the CHO cells are propagated in suspension.
5. The method according to claim 4 , wherein the cell culture is a chemostat suspension culture.
6. The method according to claim 1 , wherein the cells are cultivated by a method selected from the group consisting of batch-cultivation, feed-batch-cultivation, perfusion cultivation, and chemostat-cultivation.
7. The method according to claim 1 , wherein the polyamine is selected from the group consisting of cadaverine, putrescine, spermidine, spermine, agmatine, ornithine, and a combination thereof.
8. The method according to claim 7 , wherein the polyamine is putrescine.
9. The method according to claim 1 , wherein the protein hydrolysate is present in the animal protein-free cell culture medium in a total concentration ranging from about 0.05% (w/v) to about 5% (w/v) for all protein hydrolysates.
10. The method according to claim 1 , wherein the protein hydrolysate is derived from a plant selected from the group consisting cereals and soy.
11. The method according to claim 1 , wherein the resultant concentration of the polyamine in the animal protein-free cell culture medium ranges from about 2 mg/L to about 8 mg/L.
12. The method according to claim 1 , wherein the resultant concentration of the polyamine in the animal protein-free cell culture medium ranges from about 2 mg/L to about 5 mg/L.
13. The method according to claim 1 , wherein the polyamine originates from a source other than a protein hydrolysate.
14. The method according to claim 1 , wherein the cells express a target protein.
15. The method according to claim 14 , wherein the target protein is selected from the group consisting of coagulation factor VIII, erythropoietin, Epstein Barr virus, and human antibodies.
16. The method of claim 15 , wherein the target protein is coagulation factor VIII.
17. The method according to claim 14 , wherein the cell/target protein combination is selected from the group consisting of CHO cells/coagulation factor VIII, BHK cells/erythropoietin, Epstein Barr virus transformed, and immortalized human B cells/human antibodies.
18. The method according to claim 14 , further comprising the step of isolating the target protein from the animal protein-free cell culture medium.