Enzymatically produced cellulose
View Patent ↗Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
1. An isolated composition comprising:
(i) insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15, and
(ii) a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6.
2. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of at least 17.
3. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 50.
4. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 30.
5. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 50.
6. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 30.
7. The isolated composition of claim 1 , wherein the insoluble cellulose has a cellulose II crystal structure.
8. The isolated composition of claim 1 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
9. The isolated composition of claim 2 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
10. The isolated composition of claim 3 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
11. The isolated composition of claim 4 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
12. The isolated composition of claim 5 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
13. The isolated composition of claim 6 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
14. The isolated composition of claim 1 , wherein the cellodextrin phosphorylase enzyme is a Vibrio species cellodextrin phosphorylase enzyme or a Ruminococcus species cellodextrin phosphorylase enzyme.