Multisignal reagents for labeling analytes
Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
1. A DNA oligonucleotide having a 5′ end and a 3′ end, comprising:
a first member of a first binding pair attached at the 5′ end of the DNA oligonucleotide, wherein the first binding pair is an antigen-antibody binding pair; and
a first member of a second binding pair attached at the 3′ end of the DNA oligonucleotide, wherein the second binding pair is an antigen-antibody binding pair.
2. The DNA oligonucleotide of claim 1 , wherein the first member of the first binding pair is a protein.
3. The DNA oligonucleotide of claim 2 , wherein the first member of the first binding pair is an antibody.
4. The DNA oligonucleotide of claim 1 , wherein the first member of the second binding pair is a protein.
5. The DNA oligonucleotide of claim 4 , wherein the first member of the second binding pair is an antibody.
6. The DNA oligonucleotide of claim 3 , wherein the first member of the second binding pair is an antibody.