Functional influenza virus like particles (VLPs)
The present invention discloses and claims virus like particles (VLPs) that express and/or contains seasonal influenza virus proteins, avian influenza virus proteins and/or influenza virus proteins from viruses with pandemic potential. The invention includes vector constructs comprising said proteins, cells comprising said constructs, formulations and vaccines comprising VLPs of the inventions. The invention also includes methods of making and administrating VLPs to vertebrates, including methods of inducing substantial immunity to either seasonal and avian influenza, or at least one symptom thereof.
1. A method of stimulating an immune response in a subject comprising administering an influenza VLP, wherein the VLP comprises influenza proteins, wherein the influenza proteins consist of M1, HA and NA proteins; wherein the M1 protein is derived from a different influenza virus strain than the HA and NA proteins; wherein the influenza proteins are insect-cell expressed, and wherein the M1 protein is from an avian influenza strain.
2. The method of claim 1 , wherein the HA exhibits hemagglutinin activity and/or the NA exhibits neuraminidase activity.
3. The method of claim 1 , wherein the HA and NA proteins are from a seasonal influenza virus.
4. The method of claim 3 , wherein the seasonal influenza virus is a type A influenza virus.
5. The method of claim 3 , wherein the seasonal influenza virus is a type B influenza virus.
6. The method of claim 1 , wherein the influenza VLP comprises avian influenza HA and NA proteins.
7. The method of claim 6 , wherein the avian influenza virus HA and NA proteins are H5 and N1.
8. The method of claim 6 , wherein the avian influenza virus HA and NA proteins are H9 are N2.
9. The method of claim 1 , wherein the influenza VLP is formulated with an adjuvant.
10. The method of claim 9 , wherein the adjuvant comprises paucilamellar nonphospholipid vesicles.
11. The method of claim 1 , wherein the subject is a human.
12. The method of claim 1 , wherein the influenza VLP is administered orally, intradermally, intranasally, intramuscularly, intraperitoneally, intravenously, or subcutaneously.
13. The method of claim 1 wherein the insect cell is an Sf9 cell.
14. The method of claim 1 wherein the HA protein is selected from the group consisting of H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15 and H16.
15. The method of claim 1 wherein the NA protein is selected from the group consisting of N1, N2, N3, N4, N5, N6, N7, N8 and N9.