METHODS FOR DETECTING AND MODULATING THE EMBRYONIC-FETAL TRANSITION IN MAMMALIAN SPECIES
Aspects of the present invention include algorithms, methods and compositions related to the modulation of molecules regulating the mammalian transition from embryonic to fetal development. Methods and compositions for the use of such modulations to increase the regenerative potential in fetal and adult tissues otherwise incapable of scarless regeneration are also presented.
1 . A method for regenerating damaged or aging tissue in a subject by contacting one or more cells of the subject with one or more induced tissue regeneration (iTR) factors.
2 . The method of claim 1 , wherein the one or more iTR factors comprises a nucleic acid.
3 . The method of claim 2 , wherein the nucleic acid comprises RNA.
4 . The method of claim 1 , wherein the one or more iTR factors comprises an anti-Mullerian hormone (AMH).
5 . The method of claim 5 , wherein one or more cells of the subject are contacted with the anti-Mullerian hormone (AMH) at a concentration of between 0.05 mM and 5 mM.
6 . The method of claim 1 , wherein the one or more iTR factors comprises a protein encoded by the GFER gene.
7 . The method of claim 5 , wherein one or more cells of the subject are contacted with the protein encoded by the GFER gene at a concentration of between 2 ng/mL and 200 ng/mL.
8 . The method of claim 1 , wherein the one or more iTR factors comprises valproic acid.
9 . The method of claim 8 , wherein one or more cells of the subject are contacted with valproic acid at a concentration of between 0.05 mM and 5 mM.
10 . The method of claim 1 , wherein the one or more iTR factors are combined with a hydrogel.
11 . The method of claim 1 , wherein the iTR factors increase GFER protein levels and decrease COX7A1 protein levels.
12 . The method of claim 1 , wherein the iTR factors increase expression of LIN28A.
13 . The method of claim 1 , further comprising increasing the expression of telomerase in the one or more cells of the subject.
14 . The method of claim 13 , wherein administration of the TERT gene to the one or more cells of the subject increases expression of telomerase.
15 . The method of claim 1 , wherein the iTR factors increase expression of LIN28A and increase expression of telomerase.
16 . The method of claim 15 , wherein the subject is a human.
17 . A method for repairing damaged or aging tissue in a subject by inducing an embryonic pattern of gene expression in one or more cells of the subject.
18 . A kit for regenerating damaged or aging tissue in a subject, the kit comprising one or more of AMH, GFER protein and valproic acid iTF factors.
19 . The kit of claim 17 , wherein the iTR factors are combined with a hydrogel.
20 . A method for regenerating tissue in a subject by contacting one or more cells of the subject with an agent capable of inducing pluripotency, wherein pluripotency itself is not induced.