Cell separation apparatus and methods of use
Cell separation systems and methods of separating cells are disclosed. In an embodiment, a cell separation system is described that comprises a non-transitory storage device that executes a centrifugation program to separate cell volume from biologic material volume; a heating mechanism; a containment mechanism; and an assembly comprised of a single-walled centrifugation bowl. In an embodiment, methods of separating cells are disclosed whereby cells are separated by agitating a volume of biologic material and a volume digestion media to form a digested volume of biologic material; centrifuging the digested volume of biologic material; removing a portion of a resulting waste via at least one fluid outlet; isolating a different portion of the waste, and removing the concentrated cell volumes from the reservoir.
1. A cell separation system, comprising:
a non-transitory storage device comprising a plurality of logic associated with centrifugation programs, wherein execution of a centrifugation program separates a cell volume from a biologic material volume;
a heating mechanism electrically coupled to a power supply;
a containment mechanism in proximity of the heating mechanism;
an assembly removably coupled to the containment mechanism, wherein the assembly comprises:
a single-walled centrifugation bowl comprising a plurality of cell concentration areas, a reservoir, and a center column comprising a plurality of fluid lines,
a cradle, wherein the cradle comprises a plurality of apertures formed concentrically through the cradle, wherein the bowl is removably coupled to the cradle to enable free rotation of the bowl while coupled to the cradle,
an alignment mechanism coupled to the containment mechanism and the bowl to restrict movement of the center column of the bowl when the first assembly is coupled to the containment mechanism,
wherein, in a first state of the centrifugation programs, the assembly is configured to rotate around a central axis in a first direction and in a second direction in an alternating fashion and the heating mechanism is activated, and
wherein, in a second state of the centrifugation programs, the heating mechanism is deactivated and the single-walled bowl is configured to rotate in a single direction around the central axis to separate a plurality of waste from a plurality of cells.
2. The system of claim 1 , wherein the separated cells are separated in the second state of a centrifugation program into a plurality of cell concentration areas in the single-walled bowl.
3. The system of claim 1 , further comprising a third state, wherein, when the system is configured in the third state, the system is configured to drain a portion of the plurality of waste via a waste outlet.
4. The system of claim 3 , wherein, when configured in the third state, the system traps, in a waste block formed in the single-walled bowl, another portion of the plurality of waste comprising an average diameter larger than the waste outlet.
5. The system of claim 1 , wherein, when configured in a fourth state, the single-walled bowl is stationary.
6. The system of claim 1 , wherein the plurality of fluid lines comprises a waste outlet and a clean line.