Skin barrier preparation and method therefor
A method of selecting a skin barrier system suitable for infants and young children is disclosed.
1. A non-invasive in vivo method of evaluating a potential impact of a barrier system on infant skin, comprising:
a) topically applying said barrier system in a non-invasive in vivo method to adult skin;
b) topically applying a marker to said barrier system treated adult skin;
c) applying confocal analysis to measure-penetration of said marker into said barrier system treated adult skin;
d) using a computational model of adult skin penetration to visualize penetration of the marker by optimizing penetration parameters so that the model of adult skin penetration profiles match experimental data;
e) transferring the optimized penetration parameters to a computational model of infant skin; and
f) determining the penetration of the marker in the computational model of infant skin;
wherein step c) comprises following a concentration profile of said marker through the skin, wherein said concentration profile is measured using confocal Raman micro-spectroscopy (CRM) or confocal fluorescence microscopy;
wherein said penetration parameters are selected from the group consisting of skin surface concentration (Csurface), permeability coefficient for the stratum corneum (PSC) and permeability coefficient for the viable epidermis (PVE); and
wherein said penetration parameters are transferred in step e) such that the skin surface concentration (Csurface) parameter is higher in the computational model of infant skin than in the computational model of adult skin penetration, and such that the permeability coefficient for the stratum corneum (PSC) and the permeability coefficient for the viable epidermis (PVE) are the same between the two models.
2. The method of claim 1 , wherein the marker is caffeine.
3. The method of claim 1 , wherein EPISIM is employed as the computational model of adult skin penetration.
4. The method of claim 1 , wherein the computational model of adult skin penetration is an agent-based model.
5. The method of claim 1 , wherein said skin surface concentration (Csurface) parameter in the computational model of infant skin is twice that of the skin surface concentration (Csurface) parameter in the computational model of adult skin penetration.
6. The method of claim 1 , wherein said barrier system is topically applied through direct application or through application on a patch.