Mammalian cell culture-produced neublastin antibodies

1. A cell culture comprising a mammalian cell line genetically modified to express, and which expresses, a neublastin antibody polypeptide, or a fragment thereof, in a cell culture medium, wherein the neublastin antibody polypeptide comprises:

three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises amino acid residues 27 to 35 of SEQ ID NO:2, HCDR2 comprises amino acid residues 47 to 61 of SEQ ID NO:2, and HCDR3 comprises amino acid residues 97 to 111 of SEQ ID NO:2, and

three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises amino acid residues 27 to 34 of SEQ ID NO:4, LCDR2 comprises amino acid residues 46 to 56 of SEQ ID NO:4, and LCDR3 comprises amino acid residues 89 to 96 of SEQ ID NO:4.

2. The cell culture of claim 1 , wherein the neublastin antibody polypeptide comprises SEQ ID NO: 2, or a fragment thereof.

3. The cell culture of claim 1 , wherein the neublastin antibody polypeptide comprises SEQ ID NO: 4, or a fragment thereof.

4. The cell culture of claim 1 , wherein the neublastin antibody polypeptide comprises SEQ ID NO: 4, or a fragment thereof, and wherein the neublastin antibody polypeptide comprises SEQ ID NO: 2, or a fragment thereof.

5. The cell culture of claim 1 , wherein the mammalian cell line is a Chinese Hamster Ovary (CHO) cell line.

6. The mammalian cell culture of claim 1 , wherein the cells have been adapted to grow in a serum-free medium, an animal protein-free medium, or a chemically defined medium.

7. The cell culture of claim 1 , wherein the mammalian cells have been genetically modified with a polynucleotide encoding the neublastin antibody polypeptide, or a fragment thereof.

8. The mammalian cell culture of claim 1 , wherein the culture is a perfusion culture or is a fed batch culture.

9. The mammalian cell culture of claim 1 , wherein the medium is a serum-free medium, AN animal protein-free medium, or a chemically defined medium.

10. A neublastin antibody polypeptide produced in a large-scale mammalian cell culture, the culture comprising mammalian cells genetically modified to express, and which express, the neublastin antibody polypeptide, or a fragment thereto, in a mammalian cell culture, wherein the neublastin antibody polypeptide comprises:

three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises amino acid residues 27 to 35 of SEQ ID NO:2, HCDR2 comprises amino acid residues 47 to 61 of SEQ ID NO:2, and HCDR3 comprises amino acid residues 97 to 111 of SEQ ID NO:2, and

three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises amino acid residues 27 to 34 of SEQ ID NO:4, LCDR2 comprises amino acid residues 46 to 56 of SEQ ID NO:4, and LCDR3 comprises amino acid residues 89 to 96 of SEQ ID NO:4.

11. The neublastin antibody polypeptide of claim 10 , comprising a heavy chain polypeptide comprising SEQ ID NO: 2, or a fragment thereof.

12. The neublastin antibody polypeptide of claim 10 , comprising a light chain polypeptide comprising SEQ ID NO: 4, or a fragment thereof.

13. The neublastin antibody polypeptide of claim 10 , comprising a light chain polypeptide comprising SEQ ID NO: 4, or a fragment thereof, and a heavy chain polypeptide comprising SEQ ID NO: 2, or a fragment thereof.

14. The neublastin antibody polypeptide of claim 10 , wherein the mammalian cell culture comprises CHO cells expressing the neublastin antibody polypeptide, or a fragment thereof.

15. The neublastin antibody polypeptide of claim 10 , wherein the cells have been adapted to grow in serum-free medium, an animal protein-free medium, or a chemically defined medium.

16. The neublastin antibody polypeptide, or fragment thereof, of claim 10 , which has been isolated from the mammalian cell culture.

17. A pharmaceutical formulation comprising the neublastin antibody of claim 16 in a pharmaceutically acceptable carrier.