Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.
1. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a first nucleic acid-containing sample and a second nucleic acid-containing sample, wherein the module comprises:
a first lane comprising a first process chamber;
a waste chamber; and
a second lane comprising a second process chamber;
an assembly, wherein the first process chamber and the second process chamber are configured to be received by the assembly, the assembly comprising:
a magnetic assembly configured to apply a magnetic force to the first process chamber and the second process chamber, and
a heater assembly configured to apply heat to the first process chamber and the second process chamber, wherein the heater assembly and the magnetic assembly are configured to operate together,
wherein, when the first nucleic acid-containing sample and a first plurality of magnetic affinity beads are received in the first process chamber and when the second nucleic acid-containing sample and a second plurality of magnetic affinity beads are received in the second process chamber, the first nucleic acid-containing sample and the first plurality of magnetic affinity beads are configured to be heated within the first process chamber by the heater assembly and wherein the second nucleic acid-containing sample and the second plurality of magnetic affinity beads are configured to be heated within the second process chamber by the heater assembly, and
wherein the magnetic assembly is configured to simultaneously capture or move the first plurality of magnetic affinity beads in the first process chamber and the second plurality of magnetic affinity beads in the second process chamber; and
a liquid dispenser configured to perform suck and dispense operations on the first lane and the second lane.
2. The apparatus of claim 1 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane simultaneously.
3. The apparatus of claim 1 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane in parallel.
4. The apparatus of claim 1 , wherein when the first nucleic acid-containing sample is received in the first process chamber and when the second nucleic acid-containing sample is received in the second process chamber, the liquid dispenser is configured to withdraw all or substantially all of a liquid of the first nucleic acid-containing sample from the first process chamber, and all or substantially all of a liquid of the second nucleic acid-containing sample from the second process chamber after the use of the heater assembly and the magnetic assembly.
5. The apparatus of claim 1 , wherein the magnetic assembly is configured to move relative to the first and second process chambers.
6. The apparatus of claim 1 , wherein the magnetic assembly comprises a plurality of magnets.
7. The apparatus of claim 1 , wherein the heater assembly, the magnetic assembly, and the liquid dispenser are configured to be controlled by one or more processors.
8. The apparatus of claim 1 , wherein the heater assembly is configured to heat the first and second process chambers without unduly heating another portion of the lanes.
9. The apparatus of claim 1 , wherein operations by the heater assembly and the magnetic assembly are performed on liquid material in the first process chamber without transporting either the liquid material or the first process chamber to a different location to perform either heater assembly or magnetic assembly operations.
10. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a first nucleic acid-containing sample and a second nucleic acid-containing sample, wherein the module comprises:
a first lane comprising a first process chamber;
a waste chamber; and
a second lane comprising a second process chamber;
an assembly, wherein the first process chamber and the second process chamber are configured to be received by the assembly, the assembly comprising:
a magnetic assembly configured to apply a magnetic force to the first process chamber and the second process chamber, and
a heater assembly configured to apply heat to the first process chamber and the second process chamber, wherein the heater assembly comprises a heat block,
wherein, when the first nucleic acid-containing sample and a first plurality of magnetic affinity beads are received in the first process chamber and when the second nucleic acid-containing sample and a second plurality of magnetic affinity beads are received in the second process chamber, the first nucleic acid-containing sample and the first plurality of magnetic affinity beads are configured to be heated within the first process chamber by the heater assembly and wherein the second nucleic acid-containing sample and the second plurality of magnetic affinity beads are configured to be heated within the second process chamber by the heater assembly, and
wherein the magnetic assembly is configured to simultaneously capture or move the first plurality of magnetic affinity beads in the first process chamber and the second plurality of magnetic affinity beads in the second process chamber; and
a liquid dispenser configured to perform suck and dispense operations on the first lane and the second lane.
11. The apparatus of claim 10 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane simultaneously.
12. The apparatus of claim 10 , wherein the liquid dispenser is configured to perform suck and dispense operations on the first lane and the second lane in parallel.
13. The apparatus of claim 10 , wherein the magnetic assembly is configured to move relative to the first and second process chambers.
14. The apparatus of claim 10 , wherein the heater assembly, the magnetic assembly, and the liquid dispenser are configured to be controlled by one or more processors.
15. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a plurality of nucleic acid-containing samples, wherein the module comprises a plurality of lanes, each lane comprising a process chamber,
wherein the process chambers of the plurality of lanes are configured to be received by an assembly comprising:
a magnetic assembly configured to apply magnetic force to each process chamber of each lane; and
a heater assembly configured to heat each process chamber of each lane,
wherein when a nucleic acid-containing sample of the plurality of nucleic acid-containing samples and a plurality of magnetic affinity beads are received with a respective process chamber of the plurality of lanes, the nucleic acid-containing sample of the plurality of nucleic acid-containing samples and the plurality of magnetic affinity beads are configured to be heated within the respective process chamber by the heater assembly, and wherein the magnetic assembly is configured to be held adjacent to the respective process chamber to capture or move the plurality of magnetic affinity beads in the respective process chamber; and
a liquid dispenser configured to dispense the plurality of nucleic acid-containing samples into the process chambers of the plurality of lanes, respectively, wherein the liquid dispenser is also configured to withdraw a plurality of amplification-ready solutions from the process chambers of the plurality of lanes after the heater assembly applies heat and the magnetic assembly applies magnetic force, wherein the liquid dispenser is configured to perform operations on the plurality of lanes simultaneously.
16. The apparatus of claim 15 , wherein the liquid dispenser is configured to withdraw the entire solution from each process chamber.
17. The apparatus of claim 16 , wherein the heater assembly, the magnetic assembly, and the liquid dispenser are configured to be controlled by one or more processors.
18. The apparatus of claim 17 , wherein the heater assembly comprises a heat block.
19. The apparatus of claim 18 , wherein the heater assembly is configured to heat the process chambers of the plurality of lanes without unduly heating another portion of the lanes.
20. The apparatus of claim 15 , wherein the heater assembly and the magnetic assembly are configured to operate together.
21. The apparatus of claim 20 , wherein operations by the heater assembly and the magnetic assembly are performed on liquid material in the respective process chamber without transporting either the liquid material or the process chamber to a different location to perform either heater assembly or magnetic assembly operations.
22. The apparatus of claim 21 , wherein the magnetic assembly is configured to move relative to the process chambers.
23. The apparatus of claim 22 , wherein the magnetic assembly comprises a plurality of magnets.
24. A diagnostic apparatus comprising:
a module configured to extract nucleic acid from a plurality of nucleic acid-containing samples, wherein the module comprises a plurality of lanes, each lane comprising a process chamber,
wherein the process chambers of the plurality of lanes are configured to be received by an assembly comprising:
a magnetic assembly configured to apply magnetic force to each process chamber of each lane; and
a heater assembly configured to heat each process chamber of each lane,
wherein when a nucleic acid-containing sample of the plurality of nucleic acid-containing samples and a plurality of magnetic affinity beads are received with a respective process chamber of the plurality of lanes, the nucleic acid-containing sample of the plurality of nucleic acid-containing samples and the plurality of magnetic affinity beads are configured to be heated within the respective process chamber by the heater assembly, and wherein the magnetic assembly is configured to be held adjacent to the respective process chamber to capture or move the plurality of magnetic affinity beads in the respective process chamber; and
a liquid dispenser configured to dispense the plurality of nucleic acid-containing samples into the process chambers of the plurality of lanes, respectively, wherein the liquid dispenser is also configured to withdraw a plurality of amplification-ready solutions from the process chambers of the plurality of lanes after the heater assembly applies heat and the magnetic assembly applies magnetic force, wherein the liquid dispenser is configured to perform operations on the plurality of lanes in parallel.
25. The apparatus of claim 24 , wherein the liquid dispenser is configured to withdraw the entire solution from each process chamber.
26. The apparatus of claim 24 , wherein the heater assembly, the magnetic assembly, and the liquid dispenser are configured to be controlled by one or more processors.
27. The apparatus of claim 24 , wherein the heater assembly comprises a heat block.
28. The apparatus of claim 24 , wherein the heater assembly is configured to heat the process chambers of the plurality of lanes without unduly heating another portion of the lanes.
29. The apparatus of claim 24 , wherein the heater assembly and the magnetic assembly are configured to operate together.
30. The apparatus of claim 24 , wherein operations by the heater assembly and the magnetic assembly are performed on liquid material in the respective process chamber without transporting either the liquid material or the process chamber to a different location to perform either heater assembly or magnetic assembly operations.
31. The apparatus of claim 24 , wherein the magnetic assembly is configured to move relative to the process chambers.
32. The apparatus of claim 24 , wherein the magnetic assembly comprises a plurality of magnets.