Compositions, kits and related methods for the detection and/or monitoring of salmonella
Provided are compositions, kits, and methods for the identification of Salmonella. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
1. A detection oligonucleotide comprising a nucleotide sequence consisting essentially of the sequence of SEQ ID NO: 66, 67, 68, 69, or 70.
2. The detection oligonucleotide of claim 1 , wherein the oligonucleotide is fluorescently labeled.
3. The detection oligonucleotide of claim 2 , wherein the oligonucleotide comprises a quencher.
4. The detection oligonucleotide of claim 1 , wherein the oligonucleotide is a molecular torch oligonucleotide.
5. A kit comprising a plurality of reagents for detecting a Salmonella target nucleic acid, wherein the plurality of reagents comprises the detection oligonucleotide of claim 1 .
6. The kit of claim 5 , wherein the detection oligonucleotide is fluorescently labeled.
7. The kit of claim 5 , wherein the plurality of reagents further comprises one or more amplification oligonucleotides for amplifying the Salmonella target nucleic acid.
8. The kit of claim 7 , wherein the Salmonella target nucleic acid is an amplicon that comprises a sequence corresponding to bases from about 164 to about 209 of SEQ ID NO: 150.
9. The kit of claim 7 , wherein the one or more amplification oligonucleotides for amplifying the Salmonella target nucleic acid comprises a promoter oligonucleotide for isothermally amplifying the Salmonella target nucleic acid.
10. A nucleic acid complex comprising the detection oligonucleotide of claim 1 hybridized to a Salmonella target nucleic acid.
11. The nucleic acid complex of claim 10 , wherein the detection oligonucleotide is fluorescently labeled.
12. A composition comprising the detection oligonucleotide of claim 1 and one or more amplification and/or capture oligonucleotides for amplifying and/or capturing a Salmonella target nucleic acid.
13. The composition of claim 12 , wherein the detection oligonucleotide is fluorescently labeled.
14. The composition of claim 12 , further comprising one or more amplification oligonucleotides for amplifying a Salmonella target nucleic acid.
15. The composition of claim 12 , further comprising an RNA polymerase.
16. A method for detecting Salmonella in a sample, said method comprising a detecting step of detecting the presence or absence of a Salmonella target nucleic acid using the detection oligonucleotide of claim 1 .
17. The method of claim 16 , wherein the detection oligonucleotide is fluorescently labeled.
18. The method of claim 17 , wherein the detection oligonucleotide further comprises a quencher.
19. The method of claim 16 , wherein the method further comprises amplifying the Salmonella target nucleic acid using one or more amplification oligonucleotides.
20. The method of claim 16 , wherein the Salmonella target nucleic acid is an amplicon that comprises a sequence corresponding to bases from about 164 to about 209 of SEQ ID NO: 150.