C1q and HMGB1 fusion proteins and uses thereof
Fusion proteins of C1q peptides and HMBG1 A-box or HMBG1 B-box, or C1q peptides and DWESY peptide are provided, and methods of use thereof.
1. A method of cross-linking Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) and Receptor for Advanced Glycation Endproducts (RAGE) comprising contacting the LAIR-1 and RAGE with a fusion protein that binds to LAIR-1 and RAGE comprising (A) a C1q dodecamer peptide wherein the dodecamer peptide comprises KGEQGEPGAPGI (SEQ ID NO:3) or a C1q nonamer peptide wherein the nonamer peptide comprises KGEQGEPGA (SEQ ID NO:5), linked to (B) (i) a High Mobility Group Box 1 (HMGB1) A-box peptide, or (ii) a HMGB1 B-box peptide, or (iii) a DWEYS peptide,
and wherein when the fusion protein comprises the HMGB1 A-box peptide it has SEQ ID NO:10, and wherein when the fusion protein comprises the DWEYS peptide it has SEQ ID NO:11.
2. The method of claim 1 , wherein the fusion protein comprises a HMGB1 B-box peptide and the HMGB1 B-box peptide has SEQ ID NO:2.
3. The method of claim 2 , wherein the fusion protein has SEQ ID NO:7.
4. The method of claim 1 , wherein the LAIR-1 and RAGE are present on a monocyte, and wherein cross-linking LAIR-1 and RAGE mediates monocyte M2 differentiation.
5. The method of claim 1 , wherein the LAIR-1 and RAGE are contacted with SEQ ID NO:10.
6. The method of claim 1 , wherein the LAIR-1 and RAGE are contacted with SEQ ID NO:11.