Conversion of biomass to useful intermediates
An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
1. A non-naturally occurring Pseudomonas capable of producing 2-oxo-2H-pyran-4,6-dicarboxylic acid, comprising the deletion of genes encoding a protocatechuate 3,4-dioxygenase and further comprising insertion of genes encoding a protocatechuate 4,5-dioxygenase and a 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase wherein the Pseudomonas is capable of growing on at least one of a cellulose decomposition molecule or a lignin decomposition molecule.
2. The Pseudomonas of claim 1 , wherein the Pseudomonas is P. putida KT2440.
3. The Pseudomonas of claim 1 , wherein the cellulose decomposition molecule comprises a sugar molecule.
4. The Pseudomonas of claim 3 , wherein the sugar molecule is at least one of D-xylose or D-glucose.
5. The Pseudomonas of claim 1 , wherein the lignin decomposition molecule comprises an aromatic molecule.
6. The Pseudomonas of claim 5 , wherein the aromatic molecule is at least one of catechol, benzoate, phenol, guaiacol, protecatechuate, ferulate, p-coumarate, vanillate, or 4-hydroxybenzoate.
7. The Pseudomonas of claim 6 , wherein the aromatic molecule is protocatechuate.
8. The Pseudomonas of claim 1 , further comprising an exogenous gene encoding a carboxylase.
9. The Pseudomonas of claim 8 , wherein the exogenous gene encodes for AroY.