Compositions comprising bacterial strains
The invention provides compositions comprising bacterial strains for treating and preventing inflammatory and autoimmune diseases.
1. A pharmaceutical composition that comprises at least 1×10 6 CFU/g of a viable bacteria strain of the species Blautia wexlerae with respect to a total weight of the pharmaceutical composition, and a pharmaceutically acceptable excipient, diluent, or carrier,
wherein the Blautia wexlerae bacteria strain comprises a 16s rRNA gene sequence with at least 98% sequence identity to the sequence of SEQ ID NO:4, as determined by a Smith-Waterman homology search algorithm using an affine gap search with a gap open penalty of 12 and a gap extension penalty of 2;
wherein the Blautia wexlerae bacteria strain is lyophilized.
2. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is encapsulated.
3. The pharmaceutical composition of claim 1 , wherein the Blautia wexlerae bacteria strain is present in an amount that comprises from about 1×10 6 to about 1×10 11 CFU/g of the Blautia wexlerae bacteria strain with respect to a total weight of the pharmaceutical composition.
4. The pharmaceutical composition of claim 1 , wherein the Blautia wexlerae bacteria strain is present in an amount that comprises from about 1×10 8 to about 1×10 10 CFU/g of the Blautia wexlerae bacteria strain with respect to a total weight of the pharmaceutical composition.
5. The pharmaceutical composition of claim 1 , wherein the Blautia wexlerae bacteria strain comprises a 16s rRNA gene sequence with at least 99% sequence identity to the sequence of SEQ ID NO:4, as determined by a Smith-Waterman homology search algorithm using an affine gap search with a gap open penalty of 12 and a gap extension penalty of 2.
6. The pharmaceutical composition of claim 1 , further comprising a prebiotic compound.
7. The pharmaceutical composition of claim 1 , formulated for oral delivery.
8. The pharmaceutical composition of claim 1 , formulated for rectal delivery.
9. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated as a tablet, capsule, or powder.
10. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition does not comprise a therapeutically effective amount of additional bacteria strains.
11. The pharmaceutical composition of claim 1 , comprising the pharmaceutically acceptable carrier, wherein the pharmaceutically acceptable carrier is selected from the group consisting of lactose, starch, glucose, methyl cellulose, magnesium stearate, mannitol and sorbitol.
12. The pharmaceutical composition of claim 1 , further comprising a preservative, an antioxidant, or a stabilizer.
13. The pharmaceutical composition of claim 1 , further comprising an adjuvant.
14. The pharmaceutical composition of claim 1 , wherein the Blautia wexlerae bacteria strain comprises a 16S rRNA gene sequence that is the sequence of SEQ ID NO:4.
15. The pharmaceutical composition of claim 1 , wherein the Blautia wexlerae bacteria strain is the bacteria strain deposited under accession number NCIMB 42486.