Acyl-ACP reductase with improved properties
The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
1. A variant acyl-ACP reductase (AAR) polypeptide comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 80, wherein said variant AAR polypeptide comprises a mutation at an amino acid position selected from the group consisting of 40, 52, 273, 303, 344, and 346, and wherein said AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.
2. The variant AAR polypeptide of claim 1 , wherein expression of the variant AAR polypeptide in a recombinant host cell results in a higher titer of a fatty aldehyde or fatty alcohol composition as compared to a titer of a fatty aldehyde or fatty alcohol composition produced by expression of a wild type AAR polypeptide in a corresponding wild type host cell.
3. The variant AAR polypeptide of claim 2 , wherein said fatty alcohol composition is a C12, C14 or C16 fatty alcohol composition, or a combination thereof.
4. A variant acyl-ACP reductase (AAR) polypeptide selected from the group consisting of SEQ ID NOS: 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77 and 78.