Apparatus for detecting ATP in a liquid sample
An apparatus and kit for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity that is not substantially defined by space between a plurality of fibers. The sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.
1. A method, comprising:
using a sampling device to obtain a predetermined volume of a liquid sample;
wherein the sampling device comprises a sampling portion;
wherein the sampling portion is adapted to acquire a releasably retain a predetermined volume of liquid sample in one or more cavity, wherein the one or more cavity is not substantially defined by a space between a plurality of fibers, and further wherein the sampling portion comprises a base material and an external layer, the external layer comprising a material that affords a hydrophilic, hydrophobic, positively-charged, or negatively-charged surface to the sampling portion;
wherein the sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher;
contacting the predetermined volume of sample and the pH-adjusting reagent with a liquid reagent composition in a container to form a reaction mixture, wherein the liquid reagent composition comprises a luciferin; and
using a luminometer to detect light emitted from the reaction mixture;
wherein the container comprises a cuvette portion adapted to be operationally coupled to the luminometer.
2. The method of claim 1 , further comprising the step of agitating the predetermined volume of the sample and the liquid reagent composition in the container.
3. The method of claim 1 , wherein the liquid reagent composition further comprises a luciferase enzyme.
4. The method of claim 1 , wherein the step of contacting the sampling device comprising the sample with liquid reagent composition is conducted at a temperature within a range of 10° C. and 35° C., inclusive.
5. The method of claim 1 , wherein, after contacting the sampling device with liquid reagent composition, a substantially steady-state amount of light is emitted from the composition in less than 20 seconds.
6. The method of claim 1 , wherein the sampling portion comprises one or more of polyolefin, polyester, polyamide, poly(vinyl chloride), polymethyl methacrylate, polycarbonate, or nylon.