System and method for detection of target substances
A system and method includes a test container for detecting a target substance in a consumable sample, where the test container includes a test container body defining a test container top, a test container bottom opposing the test container top, a first chamber proximal the test container top, and a second chamber proximal the test container bottom, a magnetic diaphragm situated between the first chamber and the second chamber, the magnetic diaphragm obstructing flow of the consumable sample, and the magnetic diaphragm including a magnetic element embedded in the magnetic diaphragm, and a driving element geometrically complementary to the first chamber, the driving element including a consumable sample grinding feature protruding from a surface of the driving element.
1. A system with a test container for detecting a target substance in a consumable sample, the test container comprising:
a test container body defining:
a first chamber configured to receive the consumable sample, the first chamber defining a consumable reception opening and a second opening opposing the consumable reception opening, and
a second chamber defining:
a sample reception opening coextensive with the second opening of the first chamber, wherein the second chamber is configured to receive a homogenized sample from the first chamber, and
an outlet port at an inferior portion of the second chamber, wherein the second chamber is aligned with the first chamber along a longitudinal axis of the test container body;
a magnetic diaphragm situated between the first chamber and the second chamber, the magnetic diaphragm having a first configuration that retains at least a portion of the homogenized sample within the first chamber, the magnetic diaphragm comprising a magnetic element embedded in the magnetic diaphragm; and
a driving element geometrically complementary to the first chamber, the driving element comprising:
a shaft defining a shaft radius smaller than a radius of the consumable reception opening and a hollow channel terminating in a channel opening,
a head connected to a first end of the shaft, the head defining a dimension larger than the consumable reception opening, and
a consumable sample grinding feature extending from an interior surface of the hollow channel.
2. The test container of claim 1 , wherein the shaft defines an end of the shaft, wherein the end of the shaft defines the channel opening of the hollow channel, wherein the end of the shaft comprises a set of shearing protrusions extending along a longitudinal axis of the shaft, and wherein a length of the shaft with the shearing protrusions is greater than a length of the first chamber.
3. The test container of claim 2 , wherein the magnetic diaphragm defines a frangible region arranged radially inward from a perimeter of the magnetic diaphragm, the frangible region supported by an interior wall of the test container body.
4. The test container of claim 3 , wherein the magnetic diaphragm comprises:
a broad face proximal the first chamber; and
a grinding feature extending from the broad face towards the consumable reception opening.
5. The test container of claim 1 , wherein the driving element comprises a plunging element extending along a longitudinal axis of the shaft, wherein a length of the plunging element is greater than a length of the first chamber.
6. The test container of claim 5 , wherein the plunging element comprises a sharp tip, and wherein the plunging element is spring-loaded, wherein the plunging element extends from an interior of the hollow channel toward the channel opening.
7. The test container of claim 1 , wherein the second chamber houses a processing reagent.
8. The test container of claim 1 , further comprising a detection substrate extending along a longitudinal axis of the test container body, the detection substrate comprising:
a beginning region fluidly connected to the second chamber through an outlet port of the second chamber; and
an end region proximal the consumable reception opening of the first chamber.
9. The test container of claim 8 , wherein the detection substrate is aligned with the first chamber along a lateral axis of the test container body, and wherein the first chamber, second chamber, and detection substrate cooperatively define a consumable sample fluid path through the test container.