Factor VIII polymer conjugates
The invention is a proteinaceous construct comprising a Factor VIII molecule which is conjugated to a water-soluble polymer via carbohydrate moieties of Factor VIII, and methods of preparing same.
1. A composition comprising:
(a) a Factor VIII molecule; and
(b) at least one water soluble polymer bound to said Factor VIII molecule, wherein said water soluble polymer is attached to the Factor VIII via an oxidized carbohydrate moiety;
wherein said water soluble polymer is selected from the group consisting of polyethylene glycol (PEG), polysialic acid (PSA) and dextran;
wherein said water soluble polymer is linear;
wherein said water soluble polymer has a molecular weight of 2,000 to 150,000 Da;
wherein the Factor VIII is a recombinant Factor VIII;
wherein said molecule retains at least 50% of native FVIII activity; and said composition is substantially free from albumin.