PIK3CA fusions
The invention provides PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha) gene fusions, and fragments of those gene fusions. The invention further provides methods of diagnosing and treating diseases or disorders associated with PIK3CA fusions, such as conditions mediated by PIK3CA aberrant expression or activity, or overexpression of PIK3CA.
1. A method for detecting in a patient a FNDC3B:PIK3CA gene fusion, said method comprising:
a) contacting a biological sample from the patient with an oligonucleotide that hybridizes under stringent conditions to the fusion junction of the FNDC3B:PIK3CA gene fusion, and detecting hybridization between the FNDC3B:PIK3CA fusion and the oligonucleotide; or
b) sequencing or amplifying a portion of a nucleic acid from the patient and detecting the presence of a nucleotide sequence comprising at least the FNDC3B:PIK3CA fusion junction,
wherein the FNDC3B:PIK3CA fusion to be detected comprises SEQ ID NO: 3 or a portion thereof comprising the fusion junction.
2. The method of claim 1 , wherein the patient is suffering from or susceptible to a cancer.
3. The method of claim 2 , wherein the cancer is chosen from breast cancer, uterine cancer, and prostate cancer.
4. The method of claim 3 , wherein the cancer is breast cancer.
5. The method of claim 3 , wherein the cancer is uterine cancer.
6. The method of claim 3 , wherein the cancer is prostate cancer.