Full resolution color imaging of an object
The invention relates generally to both a method and apparatus for the creation of full resolution color digital images of diagnostic cassettes or objects of interest using a gray-scale digital camera or sensor combined with time sequential illumination using additive primary colors followed by post exposure digital processing. Such procedures and equipment is of significant economic value when employed in situations such as diagnostic clinical analyzers where space is limited and image quality requirements are high.
1. A method of constructing a monochrome digital image of an object of interest comprising:
optically obtaining a red, green, and blue color plane of image data using a grayscale digital camera or sensor;
selecting one color plane for subsequent manipulation;
creating a pixel matrix of the selected color plane, each element of the pixel matrix being indicative of a reflection intensity of a corresponding pixel of the selected color plane;
multiplying, element by element, the pixel matrix by a pre-defined color weighting matrix producing a weighted selected color plane; and
multiplying said weighted selected color plane by a pre-defined selected gain scalar producing a final selected color plane which is said constructed monochrome digital image.
2. The method of claim 1 , wherein the object of interest is a diagnostic cassette.
3. The method of claim 1 , wherein the selected color plane is the red color plane.
4. The method of claim 1 , wherein the selected color plane is the green color plane.
5. The method of claim 1 , wherein the selected color plane is the blue color plane.
6. The method of claim 1 , wherein the constructed monochrome digital image is of full resolution.
7. The method of claim 1 , wherein the constructed monochrome digital image is used as a digital image to determine the presence or absence of an agglutination reaction.
8. The method of claim 1 , further comprising illuminating the object of interest with light of a selected wavelength.
9. The method of claim 8 , wherein the light of the selected wavelength is one of red wavelength light, green wavelength light, or blue wavelength light.
10. The method of claim 8 , wherein the constructed monochrome digital image is used as a digital image to determine the presence or absence of an agglutination reaction.
11. A method of constructing a monochrome digital image of an object of interest comprising:
illuminating the object of interest with light of a selected wavelength;
optically obtaining a color plane of image data using a grayscale digital camera or sensor;
creating a pixel matrix of the color plane, each element of the pixel matrix being indicative of a reflection intensity of a corresponding pixel of the color plane;
multiplying, element by element, the pixel matrix by a pre-defined color weighting matrix producing a weighted selected color plane; and
multiplying the weighted selected color plane by a pre-defined selected gain scalar producing a final selected color plane which is the constructed monochrome digital image.
12. The method of claim 11 , wherein the object of interest is illuminated by at least one of a source of front illumination or a source of rear illumination.
13. The method of claim 12 , wherein the source of front illumination and/or the source of rear illumination is configured to emit red, green, and blue wavelength light.
14. The method of claim 11 , wherein the object of interest is a diagnostic cassette.
15. The method of claim 11 , wherein the constructed monochrome digital image is of full resolution.