IP Library › Granted Patent US 11,000,554
Granted Patent B2
US 11,000,554 · App. 16/291,784 · Granted May 11, 2021

Postpartum cells derived from placental tissue, and methods of making and using the same

Inventors: Anthony J. Kihm (Princeton, NJ); Ian R. Harris (Spring House, PA); Sanjay Mistry (Downingtown, PA); Alexander M. Harmon (Clinton, NJ); Darin J. Messina (Downingtown, PA); Agnieszka Seyda (North Brunswick, NJ); Chin-Feng Yi (Hillsborough, NJ); Anna Gosiewska (Skillman, NJ)
Assignee: DePuy Synthes Products, Inc.
A61K35/51A61K35/50A61K38/18A61K38/185A61K38/1808A61K38/1825A61K38/1833A61K38/1841A61K38/1858A61K38/1866A61K38/1891A61K38/19A61K38/204A61K38/2053A61K38/27C12N5/0605C12N5/0606C12N5/0607A61K35/12C12N2500/32C12N2500/34C12N2500/44C12N2500/90C12N2500/95C12N2501/12C12N2501/21C12N2501/23C12N2502/02C12N2506/02C12N2506/03C12N2509/00C12N2533/50
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Quick Facts
Patent No.
US 11,000,554
App. No.
16/291,784
Granted
May 11, 2021
Kind
B2
Abstract

Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications.

Claims (25)

1. A homogeneous population of cells isolated from placenta, wherein the homogeneous population of cells produces CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, HLA-A,B,C, and granulocyte chemotactic protein-2 (GCP-2); does not produce CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G or HLA-DR-DP,DQ; and expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell increased levels of oxidized low density lipoprotein receptor 1 mRNA and renin as detected by gene chip array or polymerase chain reaction.

2. The cell population of claim 1 , wherein the cell population further has the following characteristics:

(a) secretion of monocyte chemotactic protein 1 (MCP-1), interleukin-6 (IL-6), interleukin 8 (IL8), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), heparin-binding epidermal growth factor (HB-EGF), brain-derived neurotrophic factor (BDNF), tissue inhibitor of matrix metalloproteinase 1 (TIMP1), thrombopoietin (TPO), macrophage inflammatory protein 1alpha (MIP1a), Rantes (regulated on activation, normal T cell expressed and secreted), thymus and activation-regulated chemokine (TARC), and Eotaxin; and

(b) lack of secretion of fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), angiopoietin 2 (ANG2), platelet derived growth factor (PDGF-bb), transforming growth factor beta2 (TGFbeta2), macrophage inflammatory protein 1beta (MIP1b), 1309, and macrophage-derived chemokine (MDC), as detected by ELISA.

3. The cell population of claim 1 , wherein the cell population lacks production of GRO-alpha and oxidized low density lipoprotein receptor, as detected by flow cytometry.

4. The cell population of claim 1 , wherein the cell population can undergo at least 40 population doublings in culture.

5. The cell population of claim 1 , wherein the isolated homogeneous cell population is identified by ATCC Accession No. PTA-6074.

6. The cell population of claim 1 , wherein the isolated homogeneous cell population is identified by ATCC Accession No. PTA-6075.

7. The cell population of claim 1 , wherein the isolated homogeneous cell population is identified by ATCC Accession No. PTA-6079.

8. A composition comprising a homogeneous population of cells isolated from placental tissue placenta, wherein the homogeneous population of cells produces CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, HLA-A,B,C, and granulocyte chemotactic protein-2 (GCP-2); does not produce CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G or HLA-DR-DP,DQ; and expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell increased levels of oxidized low density lipoprotein receptor 1 mRNA and renin as detected by gene chip array or polymerase chain reaction.

9. The composition of claim 8 , wherein the cell population further has the following characteristics:

(a) secretion of monocyte chemotactic protein 1 (MCP-1), interleukin-6 (IL-6), interleukin 8 (IL8), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), heparin-binding epidermal growth factor (HB-EGF), brain-derived neurotrophic factor (BDNF), tissue inhibitor of matrix metalloproteinase 1 (TIMP1), thrombopoietin (TPO), macrophage inflammatory protein 1alpha (MIP1a), Rantes (regulated on activation, normal T cell expressed and secreted), thymus and activation-regulated chemokine (TARC), and Eotaxin; and

(b) lack of secretion of fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), angiopoietin 2 (ANG2), platelet derived growth factor (PDGF-bb), transforming growth factor beta2 (TGFbeta2), macrophage inflammatory protein 1beta (MIP1b), 1309, and macrophage-derived chemokine (MDC), as detected by ELISA.

10. The composition of claim 8 , wherein the cell population lacks production of GRO-alpha and oxidized low density lipoprotein receptor, as detected by flow cytometry.

11. The composition of claim 8 , wherein the cell population can undergo at least 40 population doublings in culture.

12. The composition of claim 8 , wherein the isolated homogeneous cell population is identified by ATCC Accession No. PTA-6074.

13. The composition of claim 8 , wherein the isolated homogeneous cell population is identified by ATCC Accession No. PTA-6075.

14. The composition of claim 8 , wherein the isolated homogeneous cell population is identified by ATCC Accession No. PTA-6079.

15. A matrix comprising a homogeneous population of cells isolated from placenta, wherein the homogeneous population of cells produces CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, HLA-A,B,C, and granulocyte chemotactic protein-2 (GCP-2); does not produce CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G or HLA-DR-DP,DQ; and expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell increased levels of oxidized low density lipoprotein receptor 1 mRNA and renin as detected by gene chip array or polymerase chain reaction.

16. The matrix of claim 15 , wherein the cell population further has the following characteristics:

(a) secretion of monocyte chemotactic protein 1 (MCP-1), interleukin-6 (IL-6), interleukin 8 (IL8), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), heparin-binding epidermal growth factor (HB-EGF), brain-derived neurotrophic factor (BDNF), tissue inhibitor of matrix metalloproteinase 1 (TIMP1), thrombopoietin (TPO), macrophage inflammatory protein 1alpha (MIP1a), Rantes (regulated on activation, normal T cell expressed and secreted), thymus and activation-regulated chemokine (TARC), and Eotaxin; and

(b) lack of secretion of fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), angiopoietin 2 (ANG2), platelet derived growth factor (PDGF-bb), transforming growth factor beta2 (TGFbeta2), macrophage inflammatory protein 1beta (MIP1b), 1309, and macrophage-derived chemokine (MDC), as detected by ELISA.

17. The matrix of claim 15 , wherein the cell population lacks production of GRO-alpha and oxidized low density lipoprotein receptor, as detected by flow cytometry.

18. The matrix of claim 15 , wherein the cell population can undergo at least 40 population doublings in culture.

19. The matrix of claim 15 , wherein the matrix comprises a nonwoven scaffold comprising a copolymer of glycolic and lactic acids, a 35/65 PCL/PGA foam, an in situ polymerizable gel, or a self-assembling peptide hydrogel.