Methods and devices for sequencing nucleic acids in smaller batches
The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
1. A method, comprising:
1) providing an automated nucleic acid sequencing system, comprising
a) flow cells comprising nucleic acid to be sequenced in cycles, said flow cells mounted on
b) a moveable support controlled by a
c) user interface, said support configured to move flow cells to
d) reagent delivery stations, each station in fluidic communication with
e) reagent reservoirs configured to supply extension reagents including fluorescent nucleotide analogues,
f) an imaging platform configured to detect said fluorescent nucleotide analogues, and
g) a transfer means configured for moving at least one of said flow cells from said moveable support to said imaging platform; and
2) removing a flow cell from a first position on said moveable support and repositioning said flow cell in a second position on said moveable support, said second position being different from said first position.
2. The method of claim 1 , wherein said moveable support is a carousel.
3. The method of claim 1 , further comprising an actuator configured for raising said moveable support so as to disconnect said flow cells from said stations.
4. The method of claim 1 , wherein removing said flow cell comprises off-loading said flow cell from the moving support at a first position, and said repositioning comprises loading said flow cell back onto the moving support at a second position.
5. The method of claim 1 , wherein said second position allows the flow cell to return to the same cycle without passing through non-functional stations.
6. The method of claim 1 , wherein said second position allows the flow cell to start a new sequencing cycle without passing through non-functional stations.