System for accelerating reagent reactions in a disposable cartridge
A diagnostic assay system for accelerating reagent reactions comprising a base portion receiving a disposable cartridge and a cover portion moveably mounted to the base such that the disposable cartridge may be installed and removed from the base portion. The disposable cartridge includes at least one assay chamber for receiving an assay fluid, and a cartridge body including a syringe barrel operative to move assay fluids into and out of the assay chambers. The diagnostic assay system also includes a heater proximal the at least one fluid assay-containing cavity, operational when the cover portion is in a closed position and configured to heat the assay fluid in one of the assay chambers to accelerate reagent reactions in the disposable cartridge.
1. A diagnostic assay system, including:
a base portion receiving a disposable cartridge;
a cover portion moveably mounted to the base portion such that the disposable cartridge is accessible when the cover is moved to an open position, the disposable cartridge including a cartridge body and a rotor, the cartridge body having a syringe barrel operative to move assay fluids into and out of assay chambers formed in the rotor of the disposable cartridge; and
a heating element disposed about at least a portion of the syringe barrel to heat assay fluids therein and accelerate reagent reactions in the disposable cartridge.
2. The diagnostic assay system of claim 1 wherein the heating element comprises a first heating segment disposed in the base portion to heat an underside portion of the syringe barrel.
3. The diagnostic assay system of claim 1 wherein the heating element comprises a second heating segment disposed in the cover portion to heat an upper portion of the syringe barrel.
4. The diagnostic assay system of claim 1 wherein the heating element comprises first and second heating segments disposed in the base and cover portions, respectively, to heat the underside and upper portions of the syringe barrel.
5. The diagnostic assay system of claim 1 wherein the heating element comprises a conductive sleeve circumscribing the syringe barrel.
6. The diagnostic assay system of claim 1 wherein the heating element includes a radio frequency heater.
7. The diagnostic assay system of claim 5 wherein the conductive sleeve is tuned to a predetermined Radio Frequency (RF) such that an RF generator can transmit RF energy to excite and heat the conductive sleeve.
8. The diagnostic assay system of claim 1 wherein the heating element is operative to heat the assay fluids to a temperature within a range of between fifty degrees Celsius (50° C.) to ninety degrees Celsius (90° C.).
9. The diagnostic assay system of claim 1 wherein the heating element is operative to heat the assay fluids to a temperature within a range of between sixty-five degrees Celsius (65° C.) to eighty degrees Celsius (80° C.).
10. The diagnostic assay system of claim 1 wherein the cover portion is mounted to the base by a four-bar linkage.
11. The diagnostic assay system of claim 1 wherein the cover portion is mounted to the base by an inclined guide and moves along the guide from an open to a closed position.
12. The diagnostic assay system of claim 11 wherein, in the open position, the disposable cartridge may be installed into the base portion of the assay system.
13. A diagnostic assay system, comprising:
a base portion receiving a disposable cartridge;
a cover portion movably mounting to the base from an open to a closed position such that the disposable cartridge may be received by the base portion, the disposable cartridge comprising a rotor having a plurality of chambers, and a cartridge body having a syringe barrel operative to move an assay fluid into and out of the assay chambers; and
a heater proximal to at least one chamber, operational when the cover portion is in a closed position and configured to heat the assay fluid in one of the assay chambers to accelerate reagent reactions in the disposable cartridge.
14. The diagnostic assay system of claim 13 wherein the heater circumscribes the syringe barrel to heat assay fluids therein.
15. The diagnostic assay system of claim 13 wherein the heater comprises a first and a second segments, the first segment disposed in the base portion and circumscribing a first arcuate portion of the syringe barrel, and the segment disposed in the cover portion and circumscribing a second arcuate portion of the syringe barrel.
16. The diagnostic assay system of claim 13 wherein the heater is operative to heat the assay fluids to a temperature within a range of between fifty degrees Celsius (50° C.) to ninety degrees Celsius (90° C.).
17. The diagnostic assay system of claim 13 further comprising a thermocouple disposed in combination with the heater to provide temperature feedback to a controller operative to control the temperature of the assay fluid in the syringe barrel.
18. The diagnostic assay system of claim 13 wherein the heater includes a radio frequency heater.
19. The diagnostic assay system of claim 13 wherein the cover portion is mounted to the base by an inclined guide and moves along the guide from an open to a closed position.
20. The diagnostic assay system of claim 13 wherein the cover portion is mounted to the base by a four-bar linkage.