Parallel multi-region imaging device
The parallel multi-region imaging device includes a multi-focus generation module, a spatial demodulation module, and a detection module. The multi-focus generation module is configured to modulate illumination light to generate a plurality of focuses in an object-side, and to form a plurality of different illumination regions, thereby generating multiple paths of signal light through a sample to be imaged. The spatial demodulation module is configured to make spatial energy distribution of each path of signal light no longer overlap or make an overlapping region smaller than a target requirement. The detection module is configured to independently detect each path of signal light passing through the spatial demodulation module, so as to implement parallel multi-region imaging.
1. A parallel multi-region imaging device, comprising: a multi-focus generation module, a spatial demodulation module, and a detection module;
the multi-focus generation module being configured to modulate illumination light to generate a plurality of focuses in an object-side of an objective lens, and to form a plurality of different illumination regions, thereby generating multiple paths of signal light through a sample to be imaged;
the spatial demodulation module being configured to make spatial energy distribution of each path of signal light no longer overlap or make an overlapping region smaller than a target requirement; and
the detection module being configured to independently detect each path of signal light passing through the spatial demodulation module, so as to implement parallel multi-region imaging,
the parallel multi-region imaging device further comprising a spectroscope between the multi-focus generating module and the spatial demodulation module, wherein the spectroscope is configured to spatially separate the illumination light and signal light returned from the sample to be imaged, so that the illumination light output by the multi-focus generation module is propagated to the sample to be imaged and meanwhile the signal light returned from the sample to be imaged is propagated to the spatial demodulation module,
wherein a beam splitting method of the spectroscope includes splitting based on wavelength, splitting based on polarization state, splitting based on spatial position, and attenuation type splitting.
2. The parallel multi-region imaging device according to claim 1 , further comprising a scanning module behind the spectroscope,
wherein the scanning module is configured to change a propagation direction, a divergence degree or a convergence degree of a beam, so that a focus of the object-side of the objective lens or a one-dimensionally focused line changes position in space, thereby performing scanning.
3. The parallel multi-region imaging device according to claim 2 , wherein a scanning member in the scanning module includes reflective type, transmissive type, or diffractive type.
4. The parallel multi-region imaging device according to claim 1 , further comprising a relay optical path,
wherein the relay optical path is configured to implement pupil matching between the scanning module and the objective lens, so as to enable the illumination light to enter the objective lens to illuminate the sample to be imaged within a target scanning range of the scanning module.
5. The parallel multi-region imaging device according to claim 1 , further comprising a relay optical path, wherein the relay optical path is configured to implement pupil matching between the scanning module and the objective lens, so as to enable the illumination light to enter the objective lens to illuminate the sample to be imaged within a target scanning range of the scanning module.
6. The parallel multi-region imaging device according to claim 1 , further comprising a relay optical path, wherein the relay optical path is configured to implement pupil matching between the scanning module and the objective lens, so as to enable the illumination light to enter the objective lens to illuminate the sample to be imaged within a target scanning range of the scanning module.
7. The parallel multi-region imaging device according to claim 2 , further comprising a relay optical path, wherein the relay optical path is configured to implement pupil matching between the scanning module and the objective lens, so as to enable the illumination light to enter the objective lens to illuminate the sample to be imaged within a target scanning range of the scanning module.
8. The parallel multi-region imaging device according to claim 3 , further comprising a relay optical path, wherein the relay optical path is configured to implement pupil matching between the scanning module and the objective lens, so as to enable the illumination light to enter the objective lens to illuminate the sample to be imaged within a target scanning range of the scanning module.