Device for detecting algae concentration using first derivative of visible light absorbance
Monitoring and detection of algae in surface water and wastewater is of significant importance, yet there is currently no quick and simple method to achieve this. The present work illustrates a new method to determine the concentration of algae in water and wastewater samples using spectrophotometry, the first derivative of absorbance, and a smoothing technique applied to the first derivative of absorbance (e.g. Savitzky-Golay). The relationship between algal concentration and absorbance for three types of water samples (distilled, surface, and wastewater) was determined in the visible wavelength range, and the effect of using the first derivative of absorbance method on improving algal concentration detection limit was established. Using the first derivative of absorbance method improves algal detection limits, reduces the effect of background absorbance and the resolution of overlapping spectra. The presence of algae in water can cause a number of problems, and the method presented here can be used to effectively monitor algal concentration in various types of water samples and provide necessary information for decision making purposes.
1. A device for detecting algae in water, comprising:
(i) a light source to generate light beams of electromagnetic radiation in the visible light spectrum;
(ii) a flow cell containing a water sample including windows to allow said light beams to transmit through said flow cell and water sample;
(iii) a spectrometer unit that receives said transmitted light beams penetrating said sample in said flow cell, and resolves said incident light beams into a specific visible absorbance spectrum pertaining to said sample;
(iv) a processor connected to said spectrometer unit programmed to:
a. compute the derivative of the visible absorbance spectrum through said flow cell;
b. compute from the derivative spectra the concentration of varying types of algae.
2. A device for detecting algae according to claim 1 wherein said device can be operated to measure the concentration of algae in distilled water, surface water, or wastewater.
3. A device for detecting algae according to claim 1 wherein said processor is programmed to apply a smoothing technique to improve the signal to noise ratio in the visible absorbance spectrum.
4. A device for detecting algae according to claim 3 wherein said smoothing technique is a Savitzky-Golay smoothing technique.
5. A device for detecting algae according to claim 1 wherein samples can be directly placed into said spectrometer unit for analysis with no additional sample preparation requirements.
6. A device for detecting algae according to claim 1 wherein said light source is a tungsten lamp.
7. A device for detecting algae according to claim 1 wherein said light source is an LED based light source.
8. A device for detecting algae according to claim 1 wherein said spectrometer unit is a photodiode array based spectrometer.
9. A device for detecting algae according to claim 1 wherein said spectrometer unit is a monochromator.
10. A device for detecting algae according to claim 1 wherein said device could be immersed in a sample water such that the sample water is free to flow in and out of a substantially open flow cell area.
11. A device for detecting algae according to claim 1 wherein said flow cell allows sample water to flow through either continuously or in successive batches from a sample source to a sample drain.