Sulfobetaine-based protein aggregation inhibitor for use in preventing aggregation of a protein
The present invention provides a protein aggregation inhibitor for use in preventing aggregation of a protein, containing a crosslinked polymer obtained by polymerizing polymerizable polymer components containing a sulfobetaine polymer obtained by polymerizing monomer components containing a sulfobetaine monomer, the sulfobetaine monomer, and a crosslinkable monomer.
1. A protein aggregation inhibitor for use in preventing aggregation of a protein, the inhibitor comprising a crosslinked polymer obtained by polymerizing polymerizable polymer components comprising
a sulfobetaine polymer obtained by polymerizing monomer components comprising a sulfobetaine monomer,
the sulfobetaine monomer, and
a crosslinkable monomer,
wherein the sulfobetaine monomer is represented by the formula (I):
wherein R 1 is a hydrogen atom or a methyl group, R 2 is an alkylene group having 1-4 carbon atoms, R 3 is an alkyl group having 1-4 carbon atoms, R 4 is an alkylene group having 1-4 carbon atoms, and X is an —NH— group or an —O— group.
2. The protein aggregation inhibitor according to claim 1 , wherein the monomer components further comprise a thiocarbonylthio group-containing compound having at least one carboxyl group.
3. The protein aggregation inhibitor according to claim 1 , wherein the polymerizable polymer components further comprise a hydrophobic monomer.
4. A method of producing a protein aggregation inhibitor for use in preventing aggregation of a protein and comprising a crosslinked polymer, the method comprising
polymerizing monomer components comprising a sulfobetaine monomer, and
polymerizing polymerizable polymer components comprising the resulting sulfobetaine polymer, the sulfobetaine monomer, and a crosslinkable monomer,
wherein the sulfobetaine monomer is represented by the formula (I):
wherein R 1 is a hydrogen atom or a methyl group, R 2 is an alkylene group having 1-4 carbon atoms, R 3 is an alkyl group having 1-4 carbon atoms, R 4 is an alkylene group having 1-4 carbon atoms, and X is an —NH— group or an —O— group.
5. The method according to claim 4 , wherein the monomer components are polymerized in the presence of a thiocarbonylthio group-containing compound having at least one carboxyl group.
6. The method according to claim 4 , wherein the polymerizable polymer components further comprise a hydrophobic monomer.