Increasing plant growth and yield by using a phenylalanine ammonia lyase sequence
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding phenylalanine ammonia lyase (PAL) proteins, polypeptides encompassing PAL proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
1. A method for increasing crop yield comprising transforming a plant with at least one phenylalanine ammonia lyase (PAL) protein-encoding sequence that encodes a protein having at least 95% identity with SEQ ID NO: 2, wherein the PAL protein-encoding sequence is expressed from a promoter selected from the group consisting of SEQ ID Nos: 3 and 5, and wherein the PAL protein-encoding sequence encodes a PAL protein having PAL enzyme activity.
2. A plant having stably incorporated into its genome a phenylalanine ammonia lyase (PAL) protein-encoding sequence that encodes a protein having at least 95% identity with SEQ ID NO: 2, wherein the PAL protein-encoding sequence is expressed from a promoter selected from the group consisting of SEQ ID Nos: 3 and 5, and wherein the PAL protein-encoding sequence encodes a PAL protein having PAL enzyme activity.
3. A seed of the plant of claim 2 , wherein said seed comprises said PAL protein-encoding sequence expressed from a promoter selected from the group consisting of SEQ ID NO: 3 and 5.
4. The plant of claim 2 wherein said plant is a monocot.
5. The plant of claim 2 wherein said plant is a dicot.
6. A DNA construct comprising, in operable linkage,
a. A promoter selected from the group consisting of SEQ ID Nos: 3 and 5, and
b. A nucleic acid sequence encoding a PAL protein-encoding sequence that encodes a protein having at least 95% identity with SEQ ID NO: 2.
7. The method of claim 1 , wherein the at least one PAL protein-encoding sequence has at least 95% identity with SEQ ID NO: 1.
8. The plant of claim 2 , wherein the PAL protein-encoding sequence has at least 95% identity with SEQ ID NO: 1.
9. The DNA construct of claim 6 , wherein the PAL protein-encoding sequence has at least 95% identity with SEQ ID NO: 1.