METHOD FOR THE FERMENTATIVE PRODUCTION OF METHIONINE OR ITS HYDROXY ANALOG FORM BY MICROORGANISMS COMPRISING GENES CODING SUGAR PHOSPHOTRANSFERASE SYSTEM (PTS)
The present invention relates to a new method for the production of methionine or its hydroxy analog form by conversion of a source of carbon in a fermentative process comprising culturing a microorganism genetically modified for the production of methionine orits hydroxy analog form, wherein said microorganism comprises functional genes coding PTS carbohydrate utilization system and wherein the expression of proteins regulated the expression of phosphoenolpyruvate synthase (PPS) is down-regulated. The present invention also relates to the genetically modified microorganism used in the method of the invention.
1 . A method for the production of methionine or its hydroxy analog form by conversion of a source of carbon in a fermentative process, the method comprising the following:
culturing a microorganism genetically modified for the production of methionine or its hydroxy analog form in a culture medium comprising a carbohydrate as a source of carbon, and
recovering methionine or its hydroxy analog form from the culture medium,
wherein said genetically modified microorganism comprises functional genes coding for a PTS carbohydrate utilization system, and
wherein in said genetically modified microorganism, an expression of bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase protein regulating an expression of phosphoenolpyruvate synthase (PPS) is decreased.
2 . The method of claim 1 , wherein the functional genes coding for a PTS carbohydrate utilization system are heterologous to the genetically modified microorganism.
3 . The method of claim 1 , wherein the functional genes coding for a PTS carbohydrate utilization system are native to the genetically modified microorganism.
4 . The method of claim 3 , wherein the native gene coding for the bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase protein regulating the expression of the phosphoenolpyruvate synthase (PPS) is attenuated or deleted in said genetically modified microorganism.
5 . The method of claim 1 , wherein the microorganism is at least one selected from the group consisting of Enterobacteriaceae, Bacillaceae, Clostridiaceae, Streptomycetaceae, Corynebacteriaceae, Saccharomyceteceae and yeasts.
6 . The method of claim 5 , wherein the microorganism is at least one selected from the group consisting of Escherichia coli, Klebsiella pneumoniae, Thermoanaerobacterium thermosaccharolyticum, Clostridium sphenoides and Saccharomyces cerevisiae.
7 . The method of claim 1 , wherein the microorganism is E. coli.
8 . The method of claim 7 , wherein a gene ppsR coding for bifunctional ADP dependent kinase-Pi-dependent pyrophosphorylase DUF299 protein is attenuated or deleted.
9 . The method of claim 1 , wherein the microorganism is genetically modified for an enhanced production of methionine or its hydroxy analog form.
10 . A genetically modified microorganism for an enhanced production of methionine or its hydroxy analog form from a carbohydrate as a source of carbon, wherein said genetically modified microorganism comprises:
functional genes coding for a PTS carbohydrate utilization system, and
wherein, in said genetically modified microorganism, an expression of bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase protein regulating an expression of phosphenolpyruvate synthase (PPS) is decreased.
11 . The genetically modified microorganism of claim 10 , wherein the functional genes coding for a PTS carbohydrate utilization system are heterologous to the genetically modified microorganism.
12 . The genetically modified microorganism of claim 10 , wherein a gene ppsR coding for bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase DUF229 protein is attenuated or deleted.
13 . The genetically modified microorganism of claim 10 , wherein the functional genes coding for a PTS carbohydrate utilization system are native to the genetically modified microorganism.
14 . The genetically modified microorganism of claim 13 , wherein the native gene coding for the bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase protein regulating the expression of the phosphoenolpyruvate synthase (PPS) is attenuated or deleted in said genetically modified microorganism.
15 . The genetically modified microorganism of claim 10 , wherein the microorganism is at least one selected from the group consisting of Enterobacteriaceae, Bacillaceae, Clostridiaceae, Streptomycetaceae, Corynebacteriaceae, Saccharomyceteceae and yeasts.
16 . The genetically modified microorganism of claim 15 , wherein the microorganism is at least one selected from the group consisting of Escherichia coli, Klebsiella pneumoniae, Thermoanaerobacterium thermosaccharolyticum, Clostridium sphenoides and Saccharomyces cerevisiae.
17 . The genetically modified microorganism of claim 10 , wherein the microorganism is E. coli.