IL-10 RECEPTOR ALPHA HOMING ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF USE
The invention provides improved genome editing compositions and methods for editing an IL-10Rα gene. The invention further provides genome edited cells for the prevention, treatment, or amelioration of at least one symptom of, a cancer, GVHD, a transplant rejection, an infectious disease, an autoimmune disease, an inflammatory disease, or an immunodeficiency.
1 . A polypeptide comprising a homing endonuclease (HE) variant that cleaves a target site in the human interleukin 10 receptor 1 alpha (IL-10Rα) gene, wherein the polypeptide binds and cleaves the polynucleotide sequence set forth in SEQ ID NO: 9.
2 .- 15 . (canceled)
16 . The polypeptide of claim 1 , wherein the HE variant comprises at least 40 or more of the following amino acid substitutions: S24C, L26S, R28S, R30Y, N32T, K34R, S36R, V37A, G38R, S40R, E42R, G44S, Q46V, Q46I, T48G, N59S, A70T, S72A, N75G, S78Q, K80R, T82S, L138M, T143N, E145K, S159P, F168L, C180H, C180Y, F182Y, N184R, S188P, K189R, S190R, K191D, L192A, G193R, Q195Y, Q197G, V199G, S201E, T203G, K207R, Y223R, K225Y, K227Q, N228K, K229A, F232K, D236K, and V238I, in reference to an I-OnuI LHE amino acid sequence as set forth in SEQ ID NOs: 1-5, or a biologically active fragment thereof.
17 . The polypeptide of claim 1 , wherein the HE variant comprises the following amino acid substitutions: S24C, L26S, R28S, R30Y, N32T, K34R, S36R, V37A, G38R, S40R, E42R, G44S, Q46V, T48G, A70T, S72A, N75G, S78Q, K80R, T82S, L138M, T143N, S159P, F168L, C180H, F182Y, N184R, S188P, K189R, S190R, K191D, L192A, G193R, Q195Y, Q197G, V199G, S201E, T203G, K207R, Y223R, K225Y, K227Q, K229A, F232K, D236K, and V238I, in reference to an I-OnuI LHE amino acid sequence as set forth in SEQ ID NOs: 1-5, or a biologically active fragment thereof.
18 . The polypeptide of claim 1 , wherein the HE variant comprises the following amino acid substitutions: S24C, L26S, R28S, R30Y, N32T, K34R, S36R, V37A, G38R, S40R, E42R, G44S, Q46I, T48G, A70T, S72A, N75G, S78Q, K80R, T82S, L138M, T143N, E145K, S159P, F168L, C180H, F182Y, N184R, S188P, K189R, S190R, K191D, L192A, G193R, Q195Y, Q197G, V199G, S201E, T203G, K207R, Y223R, K225Y, K227Q, N228K, K229A, F232K, D236K, and V238I, in reference to an I-OnuI LHE amino acid sequence as set forth in SEQ ID NOs: 1-5, or a biologically active fragment thereof.
19 . The polypeptide of claim 1 , wherein the HE variant comprises the following amino acid substitutions: S24C, L26S, R28S, R30Y, N32T, K34R, S36R, V37A, G38R, S40R, E42R, G44S, Q46I, T48G, N59S, A70T, S72A, N75G, S78Q, K80R, T82S, L138M, T143N, E145K, S159P, F168L, C180Y, F182Y, N184R, S188P, K189R, S190R, K191D, L192A, G193R, Q195Y, Q197G, V199G, S201E, T203G, K207R, Y223R, K225Y, K227Q, N228K, K229A, F232K, D236K, and V238I, in reference to an I-OnuI LHE amino acid sequence as set forth in SEQ ID NOs: 1-5, or a biologically active fragment thereof.
20 . The polypeptide of claim 1 , wherein the HE variant comprises an amino acid sequence that is at least 80%, preferably at least 85%, more preferably at least 90%, or even more preferably at least 95% identical to the amino acid sequence set forth in any one of SEQ ID NOs: 6-8, or a biologically active fragment thereof.
21 . The polypeptide of claim 1 , wherein the HE variant comprises the amino acid sequence set forth in any one of SEQ ID NOs: 6-8, or a biologically active fragment thereof.
22 .- 23 . (canceled)
24 . The polypeptide of claim 1 , further comprising a TALE DNA binding domain.
25 .- 27 . (canceled)
28 . The polypeptide of claim 24 , wherein the TALE DNA binding domain binds the polynucleotide sequence set forth in SEQ ID NO: 11.
29 . (canceled)
30 . The polypeptide of claim 1 , further comprising a peptide linker and Trex2 or biologically active fragment thereof.
31 .- 34 . (canceled)
35 . A polynucleotide encoding the polypeptide of claim 1 or claim 24 .
36 . The polynucleotide of claim 35 , wherein the polynucleotide is an mRNA.
37 . The polynucleotide of claim 35 , wherein the polynucleotide is a cDNA.
38 . A vector comprising the polynucleotide of claim 35 .
39 .- 40 . (canceled)
41 . A human cell comprising the vector of claim 38 .
42 .- 111 . (canceled)