Rhamnolipid-producing cell having reduced glucose dehydrogenase activity
The invention relates to cells which make rhamnolipids and are genetically modified such that they have a decreased activity, compared to the wild type thereof, of a glucose dehydrogenase and to a method for producing rhamnolipids using the cells according to the invention.
1. A cell able to make at least one rhamnolipid a compound comprising general formula 1 , wherein the cell is P. putida KT2440 Aupp Agcd+pACYCATh5-{PrhaSR} [rhaSR_Ec] {rhaBAD} [rhlAB_Pa] {Talk} [araC_Ec] {ParaBAD} [rmlBDAC_Pa] {Talk}[D]], and wherein general formula (I) is
where
m=2, 1, or 0
n=1 or 0,
and R 1 is an organic radical having from 2 to 24 carbon atoms, and R 2 is is an organic radical having from 2 to 24 carbon atoms.
2. The cell according to claim 1 , wherein the rhamnolipid comprises a mixture of rhamnolipids comprising when n=1 in general formula (I) is more than 80% by weight of the rhamnolipids.
3. The cell according to claim 1 , wherein R 1 is an organic radical having from 5 to 13 carbon atoms, and R 2 is an organic radical having from 5 to 13 carbon atoms.
4. The cell according to claim 1 , wherein R 1 is selected from the group consisting of pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and (CH 2 ) o —CH 3 where o=1 to 23.
5. The cell according to claim 1 , wherein R 2 is selected from the group consisting of pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and (CH 2 ) o —CH 3 where o=1 to 23.
6. The cell according to claim 1 , wherein R 2 is selected from the group consisting of pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and (CH 2 ) o —CH 3 where o=4 to 12.
7. The cell according to claim 1 , wherein R 1 and R 2 is
derived from 3-hydroxyoctanoyl-3-hydroxyoctanoic acid, 3-hydroxyoctanoyl-3-hydroxydecanoic acid, 3-hydroxydecanoyl-3-hydroxyoctanoic acid, 3-hydroxyoctanoyl-3-hydroxydecenoic acid, 3-hydroxydecenoyl-3-hydroxyoctanoic acid, 3-hydroxyoctanoyl-3-hydroxydodecanoic acid, 3-hydroxydodecanoyl-3-hydroxyoctanoic acid, 3-hydroxyoctanoyl-3-hydroxydodecenoic acid, 3-hydroxydodecenoyl-3-hydroxyoctanoic acid, 3-hydroxydecanoyl-3-hydroxydecanoic acid, 3-hydroxydecanoyl-3-hydroxydecenoic acid, 3-hydroxydecenoyl-3-hydroxydecanoic acid, 3-hydroxydecenoyl-3-hydroxydecenoic acid, 3-hydroxydecanoyl-3-hydroxydodecanoic acid, 3-hydroxydodecanoyl-3-hydroxydecanoic acid, 3-hydroxydecanoyl-3-hydroxydodecenoic acid, 3-hydroxydecanoyl-3-hydroxytetradecenoic acid, 3-hydroxytetradecanoyl-3-hydroxydecenoic acid, 3-hydroxydodecenoyl-3-hydroxydecanoic acid, 3-hydroxydecanoyl-3-hydroxytetradecanoic acid, 3-hydroxytetradecanoyl-3-hydroxydecanoic acid, 3-hydroxydecanoyl-3-hydroxytetradecenoic acid, 3-hydroxytetradecenoyl-3-hydroxydecanoic acid, 3-hydroxydodecanoyl-3-hydroxydodecanoic acid, 3-hydroxydodecenoyl-3-hydroxydodecanoic acid, 3-hydroxydodecanoyl-3-hydroxydodecenoic acid, 3-hydroxydodecanoyl-3-hydroxytetradecanoic acid, 3-hydroxytetradecanoyl-3-hydroxydodecanoic acid, 3-hydroxytetradecanoyl-3-hydroxytetradecanoic acid, 3-hydroxyhexadecanoyl-3-hydroxytetradecanoic acid, 3-hydroxytetradecanoyl-3-hydroxyhexadecanoic acid or 3-hydroxyhexadecanoyl-3-hydroxyhexadecanoic acid.
8. The method for producing rhamnolipids, comprising the method steps of
I) contacting the cell according to claim 1 , combining these measures as appropriate, with a medium containing a carbon source
II) culturing the cell under conditions allowing the cell to make rhamnolipid from the carbon source and
III) optionally isolating the rhamnolipids made.