Fibrinogen composition, method and wound articles
Provided is a method of forming a fibrinogen hydrogel composition, the method including providing a fibrinogen hydrogel or precursor thereof, comprising fibrinogen hydrogel forming salt. The fibrinogen hydrogel forming salt concentration is greater than or equal to the threshold concentration to form a fibrinogen hydrogel. The method further includes denaturing the fibrinogen hydrogel such as by heating. The method optionally further includes combining the fibrinogen hydrogel with a carrier material. When present, the concentration of the carrier material typically ranges from 0.1 to about 50 wt.-%. The method further includes reducing the salt concentration below the threshold concentration to form a fibrinogen hydrogel.
1. A method of forming a fibrinogen hydrogel composition comprising:
providing a fibrinogen hydrogel or precursor thereof, comprising fibrinogen hydrogel forming salt at a concentration greater than or equal to the threshold concentration to form the fibrinogen hydrogel;
denaturing the fibrinogen hydrogel;
reducing the salt concentration below the threshold concentration to form the fibrinogen hydrogel.
2. The method of claim 1 , wherein the fibrinogen hydrogel precursor comprises an aqueous solution comprising fibrinogen and the fibrinogen hydrogel forming salt.
3. The method of claim 1 , wherein the fibrinogen hydrogel forming salt comprises sodium citrate optionally in combination with sodium chloride.
4. The method of claim 1 , wherein the threshold hydrogel forming salt concentration of the aqueous solution is at least 0.45 wt.-%.
5. The method of claim 1 , wherein the fibrinogen hydrogel composition further comprises a fibrinogen hydrogel plasticizer.
6. The method of claim 5 wherein the plasticizer comprises a sugar alcohol, an alkane diol, or a combination thereof.
7. The method of claim 1 , further comprising forming the fibrinogen hydrogel composition into a sheet, foam, or a plurality of pieces.
8. The method of claim 1 , wherein the step of reducing the fibrinogen hydrogel forming salt concentration comprises rinsing the hydrogel with an aqueous solution.
9. The method of claim 1 , further comprising dehydrating the fibrinogen hydrogel composition.
10. The method of claim 1 , wherein the denaturing is accomplished by heating.
11. The method of claim 9 , wherein the dehydrated denatured fibrinogen hydrogel composition has a salt concentration no greater than 20 wt.-%.
12. The method of claim 1 , further comprising forming the fibrinogen hydrogel composition into a plurality of pieces.
13. The method of claim 1 , further comprising combining the fibrinogen hydrogel with 0.1 to about 50 wt.-% of a carrier material.
14. The method of claim 13 , wherein the carrier material comprises a water soluble polymer having a Fikentscher K-value of at least K-90.
15. The method of claim 13 , wherein the carrier material comprises a polymer.
16. The method of claim 15 , wherein the polymer comprises polymerized units of N-vinyl lactam polymer.
17. The method of claim 13 , wherein the carrier material further comprises a swelling agent.
18. The method of claim 17 , wherein the swelling agent also functions as a plasticizer for the fibrinogen hydrogel.
19. The method of claim 1 , wherein reducing the salt concentration below the threshold concentration to form the fibrinogen hydrogel is before combining the fibrinogen hydrogel with a carrier material.
20. The method of claim 1 , wherein reducing the salt concentration below the threshold concentration to form the fibrinogen hydrogel is after combining the fibrinogen hydrogel with a carrier material.