Phytase production and methods of using the same
Methods and compositions are described for producing a phytase in transgenic maize plants and then incorporating parts of the transgenic maize plants in animal feed. The feed phytase enzyme displays activity across a broad pH range, and tolerance to temperatures that are often encountered during the process of preparing animal feeds. Methods of producing an animal feed that incorporate the transgenic maize plants, parts thereof or plant derived phytases, as well as methods of promoting the release of inorganic phosphate from a phytic acid in an animal, producing an animal meat, or reducing the ratio of intake of an animal feed per weight of the animal meat by feeding an animal with the animal feed incorporating transgenic maize plants are provided.
1. A maize plant or part thereof comprising one or more synthetic nucleic acids encoding a phytase and synthetic polynucleotides of SEQ ID NON: 158 and SEQ ID NO: 159, wherein the one or more synthetic nucleic acids encoding said phytase comprise a sequence with at least 90% identity to the nucleic acid sequence of SEQ ID NO: 17 and the synthetic polynucleotides of SEQ ID NO: 158 and SEQ ID NO: 159 produce an amplicon diagnostic for event PY1203 and
wherein a representative sample of seed comprising event PY1203 has been deposited under ATCC Accession No. PTA-127093.