Endotoxin free asparaginase
Disclosed herein is an endotoxin-free asparaginase enzyme. Also disclosed are methods of using the disclosed enzyme to treat subjects with a disease treatable by depletion of asparagine. For example, the disclosed endotoxin-free asparaginase enzyme is useful in the treatment or the manufacture of a medicament for use in the treatment of acute lymphoblastic leukemia (ALL) in both adults and children, as well as other conditions where asparagine depletion is expected to have a useful effect.
1. A method for treating a subject with a disease treatable by L-asparagine depletion, comprising administering to the subject a composition comprising an endotoxin-free asparaginase isolated from Rhodococcus rhodochrous DAP 96253 cells, wherein the asparaginase comprises a heteropolymer of a polypeptide having the amino acid sequence as set forth in SEQ ID NO:6 and a polypeptide having the amino acid sequence as set forth in SEQ ID NO:8, wherein the disease is selected from the group consisting of Acute Lymphoblastic Leukemia (“ALL”), acute myelocytic leukemia, acute myelomonocytic leukemia, chronic lymphocytic leukemia, non-Hodgkin's lymphoma, NK lymphoma, and pancreatic cancer.
2. The method of claim 1 , wherein the asparaginase is a recombinant asparaginase.
3. The method of claim 1 , wherein the cells have been induced to produce the asparaginase using an inducing agent selected from the group consisting of urea, methyl carbamate, methacrylamide, acetamide, cobalt, or asparagine, and combinations thereof.
4. The method of claim 1 , wherein the asparaginase is conjugated to a polyethylene glycol (PEG).
5. The method of claim 1 , wherein the disease is juvenile or adult ALL.
6. The method of claim 1 , wherein the subject has had a previous hypersensitivity to an Escherichia L asparaginase or Erwinia L-asparaginase.
7. The method of claim 1 , wherein the asparaginase is administered in a dose that depletes L-asparagine in the subject to undetectable levels for a period of at least 3 days to 10 days.