IP Library › Granted Patent US 11,001,818
Granted Patent B2
US 11,001,818 · App. 16/355,464 · Granted May 11, 2021

High fidelity restriction endonucleases

Inventors: Zhenyu Zhu (Beverly, MA); Aine Quimby (Newton, NH); Shengxi Guan (Stoneham, MA); Dapeng Sun (Arlington, MA); Yishu Huang (Shanghai, CN); Xuhui Lai (Shanghai, CN); Siu-hong Chan (Ipswich, MA); Xianghui Li (Shanghai, CN); Shuang-Yong Xu (Lexington, MA); Chunhua Zhang (Ipswich, MA)
C12N9/22C12N9/16C12Q1/34C12Q1/44C12Q1/68C12Y301/21004G01N2333/916G01N2333/922
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Quick Facts
Patent No.
US 11,001,818
App. No.
16/355,464
Granted
May 11, 2021
Kind
B2
Abstract

Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.

Claims (4)

1. A composition comprising a variant DraIII restriction endonuclease having reduced star activity, wherein the variant DraIII restriction endonuclease comprises the amino acid sequence of SEQ ID NO: 3 except for an amino acid substitution at a position selected from positions 15, 20, 34, 58, 95, 106, 108, 181 and 187 of SEQ ID NO:3.

2. The composition according to claim 1 , wherein the amino acid substitution is selected from S15A, H20A, E34A, M58A, Q95A, R106A, K108A, T181A and R187A.

3. The composition according to claim 1 , wherein the amino acid substitution is at position 181 in SEQ ID NO: 3.

4. The composition according to claim 1 , wherein the amino acid substitution is T181A.

Assignments (2)
NOTICE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Sep 27, 2023
From: NEW ENGLAND BIOLABS, INC.
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 065044/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 19, 2019
From: ZHU, ZHENYU; QUIMBY, AINE; GUAN, SHENGXI; SUN, DAPENG; CHAN, SIU-HONG; XU, SHUANG-YONG
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 049524/0250 →