Method and system for automated image analysis in cancer cells
A method of screening for the presence and/or extent of a pathology in a subject, the pathology characterized by an abnormal chromosomal component in a cell of the subject, comprising the steps of: contacting a biological sample comprising cell nuclei from said subject with, one or more distinguishable labeled probes directed to at least one chromosomal sequence that characterizes the abnormality under conditions that promote hybridization of the one or more probes to the at least one sequence, automatically obtaining a representation of the one or more distinguishable labels hybridized to the chromosomal sequences, automatically analyzing the distribution and intensity of binding of the one or more labels in the representation to determine the presence and/or extent of an abnormal chromosomal component; and automatically reporting results of the analysis; wherein the steps are carried out without intervention by a human.
1. A kit consisting of a plurality of fluorescently labeled probes wherein each probe is greater than 20 kb and individually hybridizes to a chromosomal location selected from:
(a) at least one of LRRIQ4, LRRC31, SAMD7, LOC100128164, SEC62, GPR160, PHC3, or PRKCI; and
(b) either CEP3 or CENT; and
(c) at least one of ADCY2, C5orf49, FASTKD3, MTRR, SEMA5A, SNORD123, TAS2R1, LOC285692, FAM173B, CCT5, CMBL, MARCH6, ROPNIL, DAP, or CTNND2; and
optionally, wherein the chromosomal location further consists of 20q13.
2. The kit of claim 1 , wherein (a) is at least two of LRRIQ4, LRRC31, SAMD7, LOC100128164, SEC62, GPR160, PHC3, or PRKCI.
3. The kit of claim 2 , wherein (a) is GPR160 and PHC3.
4. The kit of claim 1 , wherein (a) is at least three of LRRIQ4, LRRC31, SAMD7, LOC100128164, SEC62, GPR160, PHC3, or PRKCI.
5. The kit of claim 4 , wherein (a) is GPR160, PHC3, and PRKCI.
6. The kit of claim 4 , wherein (a) is SAMD7, LOC100128164, SEC62, GPR160, and PHC3.
7. The kit of claim 4 , wherein (a) is LRRIQ4, LRRC31, SAMD7, LOC100128164, SEC62, GPR160 and PHC3.
8. The kit of claim 4 , wherein (a) is LRRIQ4, LRRC31, and SAMD7.
9. The kit of claim 4 , wherein (a) is LRRIQ4, LRRC31, SAMD7, LOC100128164, and SEC62.
10. The kit of claim 1 , wherein (c) is SEMA5A.
11. The kit of claim 1 , wherein (c) is CTNND2.
12. The kit of claim 1 , wherein (a) is GPR160 and PHC3, (b) is CEN7 and (c) is SEMA5A.
13. The kit of claim 1 , wherein (a) is LRRIQ4, LRRC31, and SAMD7, (b) is CEN7 and (c) is SEMA5A.
14. The kit of claim 1 , wherein (b) is CEN7 and (c) is CTNND2.
15. The kit of claim 1 , wherein (b) is CEN7 and (c) is SNORD123 or TAS2R1.