Bifunctional polypeptides
A bifunctional polypeptide comprising a specific binding partner for a peptide-MHC epitope, such as an antibody or T cell receptor, and an immune effector, such as an antibody or a cytokine, the immune effector part being linked to the N-terminus of the peptide-MHC binding part.
1. A bifunctional molecule, comprising:
(i) a polypeptide binding partner, wherein the polypeptide binding partner binds specifically to a given pMHC epitope, and
(ii) an immune effector polypeptide,
wherein:
the N-terminus of the pMHC binding partner is linked to the C-terminus of the immune effector polypeptide,
the pMHC binding partner is a single chain antibody (scFv), and
the immune effector polypeptide is a single chain anti-CD3 antibody.
2. The bifunctional molecule of claim 1 , wherein the immune effector anti-CD3 scFv comprises the CDRs of SEQ ID NO: 3.
3. The bifunctional molecule of claim 2 , wherein the immune effector anti-CD3 scFv comprises SEQ ID NO: 3.
4. The bifunctional molecule of claim 1 , wherein the immune effector antibody comprises the CDRs of BMA031.
5. The bifunctional molecule of claim 1 , wherein the immune effector antibody comprises the CDRs of 12F6.