IP Library › Granted Patent US 11,413,358
Granted Patent B2
US 11,413,358 · App. 16/379,408 · Granted Aug 16, 2022

Sperm nuclei and methods of their manufacture and use

Inventors: Kenneth Michael Evans (College Station, TX); Thomas B. Gilligan (College Station, TX)
Assignee: Inguran, LLC
A61K49/0017C12N5/061G01N15/1012G01N15/14G01N21/6486G01N33/5005
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Quick Facts
Patent No.
US 11,413,358
App. No.
16/379,408
Granted
Aug 16, 2022
Kind
B2
Abstract

The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.

Claims (4)

1. A composition comprising unsorted sperm nuclei, an aggregation-reducing compound and a DNA selective dye, wherein the composition has been sonicated and reached a temperature of 45° C. or greater.

2. The composition of claim 1 , wherein the DNA selective dye is Hoechst 33342.

3. The composition of claim 1 , wherein the sperm nuclei are derived from sperm cells from one male.

4. The composition of claim 1 , wherein the aggregation-reducing compound is egg yolk.

Assignments (3)
SECURITY INTEREST Recorded Nov 24, 2025
From: INGURAN, LLC
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 073783/0924 →
SECURITY INTEREST Recorded Mar 31, 2021
From: INGURAN, LLC
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 055791/0492 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 17, 2019
From: GILLIGAN, THOMAS B.; EVANS, KENNETH MICHAEL
To: INGURAN, LLC
Reel/Frame 049775/0071 →