Glucosyltransferase enzymes for production of glucan polymers
Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DP w ) of at least 1000. This glucan polymer comprises at least 30% alpha-1,3 linkages and at least 30% alpha-1,6 linkages. Further disclosed are glucosyltransferase enzymes that synthesize poly alpha-1,3-1,6-glucan. Ether derivatives of poly alpha-1,3-1,6-glucan and methods of using such derivatives as viscosity modifiers are also disclosed.
1. A composition comprising water-insoluble poly alpha-1,3-1,6-glucan, wherein said poly alpha-1,3-1,6-glucan is a product of a glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2, and wherein the alpha-1,3 linkages and alpha-1,6 linkages of the poly alpha-1,3-1,6-glucan do not consecutively alternate with each other.
2. The composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2.
3. The composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:2.
4. The composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:2.
5. The composition of claim 1 , wherein the glucosyltransferase enzyme comprises the amino acid sequence of SEQ ID NO:2.
6. The composition of claim 1 , further comprising the glucosyltransferase enzyme.
7. The composition of claim 6 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2.
8. The composition of claim 6 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:2.
9. The composition of claim 6 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:2.
10. The composition of claim 6 , wherein the glucosyltransferase enzyme comprises the amino acid sequence of SEQ ID NO:2.
11. The composition of claim 6 , wherein the glucosyltransferase enzyme consists of an amino acid sequence that is at least 90% identical to SEQ ID NO:2.
12. The composition of claim 6 , wherein the glucosyltransferase enzyme consists of an amino acid sequence that is at least 95% identical to SEQ ID NO:2.
13. The composition of claim 6 , wherein the glucosyltransferase enzyme consists of an amino acid sequence that is at least 98% identical to SEQ ID NO:2.
14. The composition of claim 1 , wherein the composition is a personal care product, household product, industrial product, pharmaceutical product, or food product.
15. The composition of claim 1 , wherein the composition is a laundry detergent or fabric softener.
16. The composition of claim 1 , wherein the composition is an aqueous composition.