Detection devices and related methods of use
Disclosed are devices and methods for detecting analytes from a sample.
1. A device comprising:
a. a substrate comprising a surface;
b. a non-fouling polymer layer on the surface;
c. a plurality of capture regions on the non-fouling polymer layer, each comprising at least one capture agent; and
d. a plurality of labile regions on the non-fouling polymer layer, each comprising at least one detection agent and an excipient;
wherein the plurality of capture regions and the plurality of labile regions are spatially separated, and wherein the non-fouling polymer layer allows a biological fluid to move across the surface by diffusion.
2. The device according to claim 1 , wherein the non-fouling polymer layer comprises a plurality of poly(oligo(ethylene glycol)methyl methacrylate) (POEGMA) brush molecules.
3. The device according to claim 1 , wherein each of the plurality of capture regions comprises at least two different capture agents.
4. The device according to claim 1 , wherein the at least one capture agent in each capture region of the plurality of capture regions is different from the at least one capture agent in other capture regions of the plurality of capture regions.
5. The device according to claim 1 , wherein the capture agent is a biomarker, wherein the biomarker comprises a cell, a small molecule ligand, a lipid, a carbohydrate, a polynucleotide, a peptide, a protein, an antigen, or an antibody.
6. The device according to claim 1 , wherein the detection agent comprises a small molecule ligand, a lipid, a carbohydrate, a polynucleotide, a peptide, a protein, an antigen, or an antibody.
7. The device according to claim 1 , wherein the detection agent comprises a detection moiety selected from the group consisting of a chromophore, fluorophore, a radiolabel, a polynucleotide, a small molecule, an enzyme, a nanoparticle, a microparticle, a quantum dot, or an upconverter.
8. The device according to claim 1 , wherein the excipient is selected from the group consisting of a salt, a carbohydrate, a sugar, an emulsifier, a water-soluble polymer, and any combination thereof.
9. The device according to claim 8 , wherein the excipient is poly(ethylene glycol).
10. The device according to claim 1 , wherein at least one labile region further comprises heparin.
11. The device according to claim 1 , wherein the substrate comprises glass, silicon, a metal oxide, or a polymer.
12. The device according to claim 1 , further comprising a linking layer between the substrate and the non-fouling polymer layer.
13. The device according to claim 1 , further comprising a demarcating agent to demarcate a detection region on the surface or on the non-fouling polymer layer.
14. The device according to claim 13 , wherein the demarcating agent is a hydrophobic ink or wax.
15. A method of manufacturing a device according to claim 1 , the method comprising:
a. forming a non-fouling polymer layer on a surface of a substrate;
b. printing the at least one capture agent onto the non-fouling polymer layer; and
c. printing the at least one detection agent and the at least one excipient onto the non-fouling polymer layer;
wherein the at least one capture agent is printed onto the non-fouling polymer layer in a region that is spatially separated from the at least one detection agent and the at least one excipient.
16. A method of detecting a disease, biological state or a disorder in a subject comprising:
a. contacting a biological fluid obtained from the subject with the device of claim 1 for a time sufficient to allow the at least one detection agent in the labile region to solubilize;
b. detecting the presence of a detectable signal from the detection agent,
wherein the presence of a detectable signal on at least one of the plurality of capture regions on the device indicates the presence of the disease, biological state or disorder in the subject.
17. A diagnostic assay for determining a disease, a disorder, or a biological state in a subject comprising:
a. contacting a biological fluid obtained from the subject with the device of claim 1 for a time sufficient to allow the detection agent in the labile region to solubilize;
b. detecting the presence of a detectable signal from the detection agent,
wherein the presence of a detectable signal on at least one of the plurality of capture regions on the device indicates the presence of the disease, disorder, or biological state in the subject.
18. The diagnostic assay of claim 17 , wherein the assay is a point-of-care assay.
19. A kit comprising the device according to claim 1 , further comprising a set of buffers and/or reagents, and instructions.
20. The device according to claim 1 , wherein the biological fluid is selected from the group consisting of blood, serum, plasma, lymph fluid, bile fluid, urine, saliva, mucus, sputum, tears, cerebrospinal fluid (CSF), bronchioalveolar lavage, nasopharyngeal lavage, rectal lavage, vaginal lavage, colonic lavage, nasal lavage, throat lavage, synovial fluid, semen, ascites fluid, pus, maternal milk, ear fluid, sweat, and amniotic fluid.