ENGINEERED LYMPHOCYTE COMPOSITIONS, METHODS AND SYSTEMS
The present inventions provides systems and methods to manufacture genetically modified lymphocyte cells via the use of linear DNA expression amplicons, and uses of such genetically modified lymphocyte cells to treat disease. The present invention also provides for the composition of genetically modified lymphocyte cells.
1 . An engineered lymphocyte cell comprising:
a linear DNA expression amplicon, wherein said amplicon includes an expression cassette for a CAR, TCR and/or antibody.
2 . The engineered lymphocyte cell of claim 1 , wherein the lymphocyte cell is a T cell, B cell or NK cell.
3 . The engineered lymphocyte cell of claim 1 , wherein the linear DNA expression amplicon is derived from large-scale PCR.
4 . The engineered lymphocyte cell of claim 2 , wherein the linear DNA expression amplicon further comprises a centromere, telomere or origin or replication.
5 . The engineered lymphocyte cell of claim 1 , wherein the lymphocyte cell is autologous.
6 . The engineered lymphocyte cell of claim 1 , wherein the lymphocyte cell is allogeneic.
7 . The engineered lymphocyte cell of claim 6 , wherein the linear DNA expression amplicon also includes an expression cassette for a protein to reduce MHC class 1 surface expression.
8 . A method for the production of engineered lymphocyte cells, wherein the method comprises:
providing a plurality of lymphocyte cells;
assembling a linear DNA expression amplicon template expressing a desired CAR, TCR and/or antibody;
amplifying the linear DNA expression amplicon template via PCR to create a plurality of linear DNA expression amplicons;
verifying the sequence of a partial quantity of linear DNA expression amplicons via NGS; and
transfecting the plurality of lymphocyte cells with the remaining quantity of linear DNA expression amplicons.
9 . The method of claim 8 further comprising the steps of performing in vitro transcription on a partial quantity of the linear DNA expression amplicons to produce RNA and verifying the RNA sequence via NGS;
10 . The method of claim 9 further comprising the steps of performing in vitro translation on the RNA produced via in vitro translation to produce a CAR, TCR and/or antibody protein and confirming the protein structure of the produced CAR, TCR and/or antibody via mass spectrometry.
11 . The method of claim 8 wherein the plurality of lymphocyte cells are autologous.
12 . The method of claim 8 wherein the plurality of lymphocyte cells are allogeneic.
13 . The method of claim 8 wherein the linear DNA expression amplicon template is amplified via large-scale PCR.
14 . The method of claim 8 wherein the linear DNA expression amplicon further comprises a centromere, telomere or origin or replication.
15 . A method of treating cancer, said method comprising:
administering to a subject in need thereof, an engineered lymphocyte cell comprising a linear DNA expression amplicon,
wherein said amplicon includes an expression cassette for a CAR, TCR and/or antibody.
16 . The method of claim 15 , wherein the linear DNA expression amplicon is derived from large-scale PCR.
17 . The method of claim 15 , wherein the linear DNA expression amplicon further comprises a centromere, telomere or origin or replication.
18 . The method of claim 15 , wherein the engineered lymphocyte cell is a T cell.