Plant-endophyte combinations and uses therefor
View Patent ↗The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.
1. A method for preparing a corn seed comprising an endophytic bacterial population, said method comprising applying to an exterior surface of the corn seed a formulation comprising an endophytic bacterial population consisting essentially of a Paenibacillus species of bacterium comprising a 16S rRNA comprising a nucleic acid sequence consisting of SEQ ID NO:10, wherein the endophytic bacterial population is applied in an amount effective to colonize shoot tissue of a plant grown from the corn seed comprising the endophytic bacterial population.
2. The method of claim 1 , wherein the formulation further comprises at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.
3. The method of claim 1 , the formulation comprising 10{circumflex over ( )}8-10{circumflex over ( )}9 colony forming units (CFU) of the endophytic bacterial population per mL.
4. A method for conferring one or more fitness benefits to an agricultural corn plant comprising:
a. Providing a corn seed of the corn plant;
b. Contacting the exterior surface of the seed with a formulation comprising an exogenous endophytic bacterial population consisting essentially of a Paenibacillus species of bacterium comprising a 16S rRNA comprising a nucleic acid sequence consisting of SEQ ID NO:10, wherein the exogenous endophytic bacterial population is disposed on an exterior surface of the seed or seedling in an amount effective to colonize the mature plant, wherein the formulation further comprises at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient; and
c. Allowing the seed to grow to a plant under conditions that permit the endophytic bacterium to colonize the plant.
5. The method of claim 4 , wherein the one or more of the fitness benefits are selected from the group consisting of increased germination, increased biomass, increased flowering time, increased plant biomass, increased fruit or grain yield, increased biomass of the fruit or cob, and increased drought tolerance compared to a plant grown from a non-contacted seed under the same conditions.
6. The method of claim 4 , the formulation comprising 10{circumflex over ( )}8-10{circumflex over ( )}9 colony forming units (CFU) of the endophytic bacterial population per mL.
7. The method of claim 4 , wherein the fitness benefit is increased photosynthetic rates by at least 17%.
8. The method of claim 4 , wherein the fitness benefit is increased rate of seed germination by at least 20%.
9. The method of claim 4 , wherein the fitness benefit is increased root biomass.
10. The method of claim 4 , wherein the fitness benefit is increased total biomass.
11. The method of claim 4 , wherein the fitness benefit is increased stomatal conductance.
12. The method of claim 4 , wherein the fitness benefit is increased photochemical efficiency.
13. The method of claim 4 , wherein the fitness benefit is increased leaf area.
14. The method of claim 4 , wherein the fitness benefit is increased chlorophyll content.