Methods for analyzing nucleic acids from single cells
Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
1. A composition for multiplexed nucleic acid analysis, comprising:
a plurality of beads, wherein the beads are covalently attached to a plurality of oligonucleotide tags, and wherein an oligonucleotide tag of said plurality of oligonucleotide tags comprises:
(a) a first tag sequence configured to distinguish a sample polynucleotide originating from a cell from sample polynucleotides originating from other cells; and
(b) a second tag sequence configured to distinguish said sample polynucleotide from other sample polynucleotide from the same cell and having the same sequence as said sample polynucleotide.
2. The composition of claim 1 , wherein an oligonucleotide tag of said plurality of oligonucleotide tags comprises a sequencing adaptor.
3. The composition of claim 1 , wherein an oligonucleotide tag of said plurality of oligonucleotide tags comprises a capture sequence that is configured to capture said sample polynucleotides.
4. The composition of claim 3 , wherein said capture sequence is a poly-dT sequence.
5. The composition of claim 1 , wherein said plurality of oligonucleotide tags are generated by combinatorial synthesis from a defined set of subunits.
6. The composition of claim 1 , wherein the first tag sequence has a length of 8 to 20 nucleotides.
7. The composition of claim 1 , wherein the second tag sequence has a length of 8 to 20 nucleotides.
8. The composition of claim 1 , wherein said plurality of oligonucleotide tags comprises 1,000 different first tag sequences.
9. The composition of claim 8 , wherein said plurality of oligonucleotide tags comprises 10,000 different first tag sequences.
10. The composition of claim 9 , wherein said plurality of oligonucleotide tags comprises 1,000,000 different first tag sequences.
11. The composition of claim 1 , wherein said plurality of oligonucleotide tags comprises 1,000 different second tag sequences.
12. The composition of claim 11 , wherein said plurality of oligonucleotide tags comprises 10,000 different second tag sequences.
13. The composition of claim 12 , wherein said plurality of oligonucleotide tags comprises 1,000,000 different second tag sequences.