Engineered microorganisms for the production of intermediates and final products
The present disclosure relates to a non-naturally occurring microorganism that includes an endogenous genetic deletion that eliminates the expression of at least a pyruvate kinase, where the genetically modified prokaryotic microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate.
1. A non-naturally occurring Pseudomonas sp. comprising an endogenous genetic deletion that eliminates the expression of a pyruvate kinase encoded by pykF as well as genetic deletions of gcd and hexR genes, wherein the microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate and muconic acid;
and wherein the muconic acid production is greater than that in the naturally occurring Pseudomonas sp.
2. The microorganism of claim 1 , wherein the microorganism is selected from the group consisting of P. putida, P. fluorescens , and P. stutzeri.
3. The microorganism of claim 2 , wherein the microorganism is P. putida KT2440.
4. The microorganism of claim 1 , wherein the microorganism catabolizes at least one of a lignin depolymerization product, a cellulose depolymerization product, and a hemicellulose depolymerization product.
5. The microorganism of claim 4 , wherein the lignin depolymerization product comprises an aromatic compound.
6. The microorganism of claim 4 , wherein the cellulose depolymerization product comprises glucose.