IP Library › Granted Patent US 10,590,450
Granted Patent B2
US 10,590,450 · App. 16/401,166 · Granted Mar 17, 2020

Glucosyltransferase enzymes for production of glucan polymers

Inventors: Mark S. Payne (Wilmington, DE); Yefim Brun (Wilmington, DE); Hongxian He (Wilmington, DE); Thomas Scholz (Bear, DE)
Assignee: DuPont Industrial Biosciences USA, LLC
C12P19/18C08B37/0009C12N9/1048C12N9/1051C12P19/04C12Y204/01005C12Y204/01267Y02P20/52
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Quick Facts
Patent No.
US 10,590,450
App. No.
16/401,166
Granted
Mar 17, 2020
Kind
B2
Abstract

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

Claims (18)

1. A composition comprising (i) insoluble poly alpha-1,3-glucan having at least 90% alpha-1,3 glycosidic linkages, and (ii) an isolated glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4.

2. The composition of claim 1 , wherein said insoluble poly alpha-1,3-glucan has a number average degree of polymerization of at least 100.

3. The composition of claim 1 , wherein said insoluble poly alpha-1,3-glucan has at least 95% alpha-1,3 glycosidic linkages.

4. The composition of claim 1 , wherein said insoluble poly alpha-1,3-glucan has at least 97% alpha-1,3 glycosidic linkages.

5. The composition of claim 1 , wherein said insoluble poly alpha-1,3-glucan has at least 99% alpha-1,3 glycosidic linkages.

6. The composition of claim 1 , wherein said insoluble poly alpha-1,3-glucan has about 100% alpha-1,3 glycosidic linkages.

7. The composition of claim 1 , wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 93% identical to SEQ ID NO:4.

8. The composition of claim 1 , wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:4.

9. The composition of claim 1 , wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:4.

10. The composition of claim 1 , wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:4.

11. The composition of claim 1 , wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:4.

12. The composition of claim 1 , wherein a heterologous amino acid sequence of 1-300 residues is at the N-terminus and/or C-terminus of said glucosyltransferase enzyme.

13. The composition of claim 1 , wherein the composition further comprises fructose.

14. The composition of claim 1 , wherein the composition further comprises fructose, glucose, sucrose, or leucrose.

15. The composition of claim 1 , wherein the composition further comprises fructose, glucose, sucrose and leucrose.

16. The composition of claim 1 , wherein the composition does not comprise fructose.

17. The composition of claim 1 , wherein the composition does not comprise fructose, glucose, sucrose, or leucrose.

18. The composition of claim 1 , wherein the composition does not comprise fructose, glucose, sucrose and leucrose.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 28, 2020
From: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
To: NUTRITION & BIOSCIENCES USA 4, INC.
Reel/Frame 055028/0496 →